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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Parasitology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1505824

In Vitro Ovicidal Studies on Egg-parasitic Fungus Pochonia chlamydosporia and Safety Tests on Mice

Provisionally accepted
Yuan MA Yuan MA 1,2,3*Jinbao Lv Jinbao Lv 4*Lili Jiang Lili Jiang 5*Zhaobin Fan Zhaobin Fan 5*Luyao Hao Luyao Hao 1,2,3Zhengyi Li Zhengyi Li 1,2,3*Chengyu Ma Chengyu Ma 1,2,3*Rui Wang Rui Wang 1,2,3*Hongliang Luo Hongliang Luo 6*
  • 1 Inner Mongolia Agricultural University, Hohhot, China
  • 2 Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, Hohhto, China
  • 3 National Center ofTechnologyInnovation for Dairy, Hohhot, China
  • 4 Zhongnong Dong Jun Animal Diagnosis Technology (Beijing) Co., Ltd, Beijing, China
  • 5 College of Pharmacy, Heze University, Heze, Shandong Province, China
  • 6 Rui Pu Agricultural Technology Co., Ltd, Hohhot, Inner Mongolia Autonomous Region, China

The final, formatted version of the article will be published soon.

    The control of parasites infections in livestock is an ongoing concern, with parasites developing resistance to commonly used antiparasitic drugs. The current study investigated in vitro the destructive effect of the fungus Pochonia chlamydosporia on the eggs and oocysts of several equine parasites, as well as assessing the safety of the fungus in mice. S. equinus, P. equorum, Anoplocephala spp eggs and Eimeria spp. oocysts were treated with P. chlamydosporia. The prepared preparation was also administered to mice, and the physiological indexes and lesions of major tissues and organs, as well as pathological sections of tissue, were then observed. P. chlamydosporia exhibited varying degrees of efficacy in the control of S. equinus, P. equorum, Anoplocephala spp eggs and Eimeria spp.oocysts. The acute toxicity test demonstrated that there was no death or toxicity symptom observed in the mice, with no significant difference in clinical observations, such as respiration, mental state, appetite, or feces, between the control and treated mice after the feeding of the biological preparation of P. chlamydosporia. These findings suggested that administration of P. chlamydosporia would be safe to use in livestock and provided a rationale for its potential clinical application, pending further analyses.

    Keywords: biocontrol, P. chlamydosporia, Eggs, Toxicity, Mice, Parasites

    Received: 03 Oct 2024; Accepted: 23 Dec 2024.

    Copyright: © 2024 MA, Lv, Jiang, Fan, Hao, Li, Ma, Wang and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yuan MA, Inner Mongolia Agricultural University, Hohhot, China
    Jinbao Lv, Zhongnong Dong Jun Animal Diagnosis Technology (Beijing) Co., Ltd, Beijing, China
    Lili Jiang, College of Pharmacy, Heze University, Heze, 274015, Shandong Province, China
    Zhaobin Fan, College of Pharmacy, Heze University, Heze, 274015, Shandong Province, China
    Zhengyi Li, Inner Mongolia Agricultural University, Hohhot, China
    Chengyu Ma, Inner Mongolia Agricultural University, Hohhot, China
    Rui Wang, Inner Mongolia Agricultural University, Hohhot, China
    Hongliang Luo, Rui Pu Agricultural Technology Co., Ltd, Hohhot, Inner Mongolia Autonomous Region, China

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