Skip to main content

ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1503817
This article is part of the Research Topic Exploring Antinutritional Factors in Animal Feed: Implications for Health and Productivity View all 4 articles

Enzymatic hydrolysis processing of soybean meal altered its structure and in vitro protein digestive dynamics in pigs

Provisionally accepted
Dapeng  Wang Dapeng Wang 1,2,3Honglin  Du Honglin Du 1,2,3Xiuquan  Dang Xiuquan Dang 1,2,3Yufei  Zhao Yufei Zhao 1,2,3Jiaxuan  Zhang Jiaxuan Zhang 1,2,3Rujie  Liu Rujie Liu 1,2,3Zhenying  Ge Zhenying Ge 1,2,3Qingzhen  Zhong Qingzhen Zhong 1,2,3*Zewei  Sun Zewei Sun 1,2,3*
  • 1 Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun, China
  • 2 Jilin Province Key Laboratory of Animal Nutrition and Feed Science, Changchun, China
  • 3 College of Animal Science and Technology, Jilin Agricultural University, Changchun, China

The final, formatted version of the article will be published soon.

    The study evaluated the enzymatic hydrolysis processing on physicochemical properties and protein digestive dynamics of soybean meal (SBM), as well as the relationship between protein secondary structure and digestive parameters was established. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) were employed to analyze the microstructure and protein structure of the SBM and enzymatic hydrolysis processed soybean meal (ESBM). SBM and ESBM were incubated with pepsin at pH 3.5 and 39℃ for 30 min, then with pancreatin at pH 6.8 for 0 to 240 min. The in vitro protein digestive dynamics were described as the release dynamics of amino acids and low molecular weight peptides (AA_LMW). The results showed that enzymatic hydrolysis processing did not alter the chemical composition of SBM, but changed its microstructure and protein structure. After enzymatic hydrolysis processing, the size of blocky structures of SBM decreased, exhibiting a fibrous surface and a relatively loose internal structure. The β-sheet content of ESBM was lower than that of SBM (p < 0.05), while the α-helix, β-turn, and α-helix/β-sheet content was higher than that of SBM (p < 0.05). The release rates (k) of AA_LMW in SBM and ESBM were 0.0123 min -1 and 0.0733 min -1 , respectively. Enzymatic hydrolysis processing increased the CPfast content of SBM (p < 0.05) and decreased the CPslow and CPresistant contents (p < 0.05). α-helix, β-turn, and the ratio of α-helix to β-sheet were positively correlated with CPfast and k (p < 0.05) and were negatively correlated with CPslow and CPresistant (p < 0.05). β-sheet was negatively correlated with CPfast and k (p < 0.05) and was positively correlated with CPslow and CPresistant (p < 0.05). Enzymatic hydrolysis processing altered the digestive dynamics of SBM, increased the CPfast content and the release rate of AA_LMW, which might be attributed to the structure changes of SBM.

    Keywords: Enzymatic hydrolysis, Soybean meal, protein, Structure, Digestive dynamics

    Received: 01 Oct 2024; Accepted: 08 Nov 2024.

    Copyright: © 2024 Wang, Du, Dang, Zhao, Zhang, Liu, Ge, Zhong and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qingzhen Zhong, Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun, China
    Zewei Sun, Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.