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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1496067
This article is part of the Research Topic Advancements in Synthetic Microbiomes for Enhancing Animal Health View all articles

Momoridica charantia and Fermented Momoridica charantia with Leuconostoc mesenteroides MKSR changed intestinal microbial diversity indices and compositions in high-fat-cholesterol diet-fed C57BL/6 male mice

Provisionally accepted
Heewon Moon Heewon Moon 1Kangwook Lee Kangwook Lee 2Jung-Heun Ha Jung-Heun Ha 1Na Yeun Kim Na Yeun Kim 2Hyo Ri Shin Hyo Ri Shin 2Tae Jin Cho Tae Jin Cho 2Namsu Oh Namsu Oh 2Jinbong Park Jinbong Park 3Jingsi Tang Jingsi Tang 4Jae Kyeom Kim Jae Kyeom Kim 2*Misook Kim Misook Kim 1*
  • 1 Dankook University, Yongin, Gyeonggi, Republic of Korea
  • 2 Korea University, Sejong, Republic of Korea
  • 3 Kyung Hee University, Seoul, Republic of Korea
  • 4 Chinese Academy of Sciences (CAS), Beijing, Beijing, China

The final, formatted version of the article will be published soon.

    This study explores the impact of 4% Momordica charantia (MC) and 4% fermented Momordica charantia (FMC) on gut microbiota and obesity-related health outcomes in male C57BL/6 mice, a model relevant to veterinary sciences for understanding metabolic and gut health disorders in animals. Mice were assigned to four dietary regimens, including control, high-fat and high-cholesterol diet (POS), POS with 4% MC, and POS with 4% FMC (fermented with Leuconostoc mesenteroides MKSR) over 12 weeks. Fecal samples were collected for 16S rRNA sequencing to evaluate microbial diversity and composition, key factors influencing animal health. Both MC and FMC groups demonstrated significant alterations in gut microbial communities, particularly with FMC leading to a distinct shift in beta diversity indices, which could impact gut barrier functions and inflammatory responses in animals. Changes in key microbial taxa including Bacteroidetes, Verrucomicrobia, and Firmicutes were observed, along with an enhancement in the 'L-glutamate and L-glutamine biosynthesis' pathway, suggesting a functional impact on gut metabolism. These microbial shifts were associated with reduced body weight gain and liver weights, indicating potential benefits for animal health management in preventing diet-induced metabolic disorders and protective effects against diet-induced obesity. Our study highlights the potential of MC and FMC in modulating gut microbiota and mitigating obesity-related health issues.

    Keywords: Momoridica charantia, Fermentation, Leuconostoc mesenteroides MKSR, high-fat diet, Obesity, Gut Microbiota

    Received: 13 Sep 2024; Accepted: 24 Sep 2024.

    Copyright: © 2024 Moon, Lee, Ha, Kim, Shin, Cho, Oh, Park, Tang, Kim and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jae Kyeom Kim, Korea University, Sejong, 02841, Republic of Korea
    Misook Kim, Dankook University, Yongin, 448-701, Gyeonggi, Republic of Korea

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