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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1495745
This article is part of the Research Topic Mitigating Pathogen Load and AMR in Animals via Prebiotics and Probiotics View all 5 articles

Isolation and In vitro investigation on Lactic acid bacteria for potential probiotic properties from cat faeces

Provisionally accepted
Jiali Wang Jiali Wang 1Xue Yang Xue Yang 1Yi Peng Yi Peng 2Jingyi Zhang Jingyi Zhang 1Yixin Huang Yixin Huang 1Zhijun Zhong Zhijun Zhong 1Haifeng Liu Haifeng Liu 1Hualin Fu Hualin Fu 1Ziyao Zhou Ziyao Zhou 1*Guangneng Peng Guangneng Peng 1*
  • 1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, China
  • 2 College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China

The final, formatted version of the article will be published soon.

    Background: Probiotics, which are beneficial to the host, have been shown to benefit the health of cats. Lactic acid bacteria (LAB) are commonly used probiotics, but most strains used for cats are not derived from cats, leading to reduced efficacy and poor adaptation to cats. The objective was to identify LAB with promising probiotic potential specific to cats. Method: LABs were isolated from fecal samples of 20 healthy cats. Gram staining and the survival rate in the simulated gastrointestinal tract were used for preliminary screening. Candidate strains were identified by 16S rDNA sequencing, and further evaluated for adhesion ability, growth characteristics, antibacterial activity, antioxidant capacity, and safety.Results: 24 Gram-positive isolates were identified, with 10 (F1-F10) showing robust viability in the simulated gastroenteric fluid. These 10 strains exhibited excellent adhesion to Caco-2 cells and strong auto-agglutination properties. They also possessed the capacity to antagonize and aggregate pathogens (Staphylococcus aureus ATCC 25923, Salmonella Braenderup H9812, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa PAO1), Moreover, all strains demonstrated tolerance to H2O2 concentrations ranging from 0.5-2 mmol/L and the ability to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, indicating a certain level of antioxidant activity. Safety tests showed no hemolytic activity, and all but F6 were highly sensitive to antibiotics, with over 62.5% sensitivity to 16 antibiotics. Remarkably, F4 (Lactobacillus reuteri) and F10 (Lactobacillus brevis) exhibited exceptional viability in the simulated gastrointestinal tract, coupled with robust growth potential, enhanced adhesion efficiency, significant antibacterial and antioxidant properties.Our findings revealed that F4 (Lactobacillus reuteri) and F10 (Lactobacillus brevis) hold promising potential as probiotics. This research lays a solid scientific foundation for the selection and application of probiotics tailored specifically for cats.

    Keywords: cat, lactic acid bacteria strains, Probiotics, Antimicrobial activity, antioxidant capacity

    Received: 13 Sep 2024; Accepted: 19 Nov 2024.

    Copyright: © 2024 Wang, Yang, Peng, Zhang, Huang, Zhong, Liu, Fu, Zhou and Peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ziyao Zhou, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, China
    Guangneng Peng, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.