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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1493650
This article is part of the Research Topic The application of new technologies such as new vaccines, therapeutic cytokines and antibodies, and antiviral drugs in the prevention and treatment of animal infectious diseases View all 6 articles

A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets

Provisionally accepted
Yintao He Yintao He 1,2Kaiyuan Xie Kaiyuan Xie 1,2Yuan Zhongmao Yuan Zhongmao 1,2Ting Ouyang Ting Ouyang 1,2*Anran Dong Anran Dong 1,2*Bing Ling Bing Ling 1,2*Weijun Zeng Weijun Zeng 1,2Yiqi Fang Yiqi Fang 1,2*Yiwan Song Yiwan Song 1,2Lianxiang Wang Lianxiang Wang 3*Hongxing Ding Hongxing Ding 1,2*Mingqiu Zhao Mingqiu Zhao 1,2*Shaungqi Fan Shaungqi Fan 1,2*Lin Yi Lin Yi 1,2Dongfang Zhao Dongfang Zhao 1,3,4*Jinding Chen Jinding Chen 1,2*
  • 1 South China Agricultural University, Guangzhou, China
  • 2 Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
  • 3 Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China
  • 4 Yunfu Branch, Guangdong Laboratory: for Lingnan Modern: Agriculture, GuangZhou, China

The final, formatted version of the article will be published soon.

    Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46+P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines.

    Keywords: Porcine Mycoplasma pneumonia, Baculovirus expression system, Subunit Vaccines, Immunity, Co-expression of multiple promoters

    Received: 09 Sep 2024; Accepted: 21 Oct 2024.

    Copyright: © 2024 He, Xie, Zhongmao, Ouyang, Dong, Ling, Zeng, Fang, Song, Wang, Ding, Zhao, Fan, Yi, Zhao and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ting Ouyang, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Anran Dong, South China Agricultural University, Guangzhou, China
    Bing Ling, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Yiqi Fang, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Lianxiang Wang, Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China
    Hongxing Ding, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Mingqiu Zhao, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Shaungqi Fan, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, China
    Dongfang Zhao, South China Agricultural University, Guangzhou, China
    Jinding Chen, South China Agricultural University, Guangzhou, China

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