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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Livestock Genomics
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1491160
This article is part of the Research Topic Exploring the Intersection of Animal Breeding, Genetics, and Genomics in Modern Agriculture View all articles

Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes

Provisionally accepted
Wei Zhang Wei Zhang 1*Shiyin Wang Shiyin Wang 1*Liwei Yang Liwei Yang 1*Li Gao Li Gao 1*Chengcheng Ning Chengcheng Ning 1*Mengsi Xu Mengsi Xu 2Shuangyi Deng Shuangyi Deng 1*Shangquan Gan Shangquan Gan 3*
  • 1 Xinjiang Agricultural Vocational and Technical University, Changji, China
  • 2 Xinjiang Academy of Agricultural and Reclamation Sciences (XAARS), Shihezi, Xinjiang Uyghur Region, China
  • 3 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China

The final, formatted version of the article will be published soon.

    The fat tail of sheep is an adaptive trait that could help them to adapt thefacilitates their adaptation to harsh natural environments. MicroRNAs (miRNAs) have been proved demonstrated to play important crucial roles in the regulation of tail fat deposition. HereIn this study, miRNA-Seq were was used employed to investigate the expression profiles of miRNAs during different developmental stages of sheep fat tails, and elucidate the functions of differentially expressed miRNAs (DE miRNAs). A total of 350 DE miRNAs were identified, and among which 191, 60, 26, and 21 of them were extremely significantly up-regulated in tail fat tissues of fetal, lamb, hogget Altay sheep and adult Xinjiang fine wool (XFW) sheep, respectively, but were significantly down-regulated in other stages. Furthermore, we predicted a set of candidate target genes (4,476) for the top 20 DE miRNAs.4,476 candidate target genes of top 20 DE miRNAs were predicted, the Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that they involved in several adipogenesis related pathways. Further Subsequent investigations showed indicated that four DE miRNAs, could inhibit suppress the expression of peroxisome proliferator-activated receptor gamma (PPARĪ³) and phosphoinositide-3-kinase, regulatory subunit 3 (PIK3R3), and regulate the preadipocyte development of in sheep preadipocytes. Meanwhile, the lipid metabolism related genes, fatty acid binding protein (FABP3), perilipin 1 (PLIN1), adiponectin C1Q and collagen domain containing (ADIPOQ), and lipoprotein lipase (LPL), were significantly down-regulated (P<0.01). In summary, the miRNAs expression patterns of miRNAs exhibited significant fluctuations fluctuated sharply during different development periods of the fat tail, and some of them may participate the regulation of tail fat deposition by modulating the proliferation and differentiation of preadipocytes.

    Keywords: miRNA, Sheep, fat tissue, Tail, Preadipocyte, Adipogenesis

    Received: 04 Sep 2024; Accepted: 20 Nov 2024.

    Copyright: Ā© 2024 Zhang, Wang, Yang, Gao, Ning, Xu, Deng and Gan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wei Zhang, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Shiyin Wang, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Liwei Yang, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Li Gao, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Chengcheng Ning, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Shuangyi Deng, Xinjiang Agricultural Vocational and Technical University, Changji, China
    Shangquan Gan, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China

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