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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Veterinary Experimental and Diagnostic Pathology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1483990
This article is part of the Research Topic Emerging Diseases and Diagnostics in Poultry Production View all 4 articles

Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay

Provisionally accepted
Shuyu Chen Shuyu Chen Yuyi Chen Yuyi Chen Mengyan Zhang Mengyan Zhang Wenyu Zhang Wenyu Zhang Huanru Fu Huanru Fu Yu Huang Yu Huang Longfei Cheng Longfei Cheng Chunhe Wan Chunhe Wan *
  • Fujian Academy of Agricultural Sciences, Fuzhou, China

The final, formatted version of the article will be published soon.

    Duck adeno-associated Virus (DAAV) is a novel pathogen that was recently discovered in ducks. To establish a molecular detection assay for DAAV for further epidemiological investigation and pathogenic mechanism. Here, we designed specific primers and probes according to the sequence characteristics of the newly discovered DAAV and then established a TaqMan real-time PCR method (TaqMan-qPCR) for the detection of DAAV. Our data showed that the established TaqMan-qPCR for detecting DAAV had high sensitivity, with the lowest detection limit of 29.1 copies/μL. No cross reaction was found with duck circovirus (DuCV), H9N2 subtype avian influenza virus (AIV), avian Tembusu virus (ATmV). duck hepatitis A virus 1 and 3 (DHAV-1 and DHAV-3), duck adenovirus A (DAdV-A), duck adenovirus 3 (DAdV-3), or duck enteritis virus (DEV). The repeatability was excellent, with the coefficients of variation of repeated intragroup and intergroup tests ranging from 0.12-0.21% and 0.62-1.42%, respectively. Seventy-eight clinical samples collected from diseased or deceased ducklings were tested. The results showed that the DAAV positive rate was 21.79%, and a triple infection (DAAV+MDPV+GPV) was found. These data provide technical support for further molecular epidemiological surveillance and pathogenic mechanism studies of DAAV infection.

    Keywords: Duck adeno-associated virus, DAAV, TaqMan, qPCR, epidemiological surveillance

    Received: 21 Aug 2024; Accepted: 29 Oct 2024.

    Copyright: © 2024 Chen, Chen, Zhang, Zhang, Fu, Huang, Cheng and Wan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chunhe Wan, Fujian Academy of Agricultural Sciences, Fuzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.