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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Epidemiology and Economics
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1483653
This article is part of the Research Topic Pathogens at the interface of animals in close contact with humans: risks and benefits, with special regard to immunosuppressed people View all 5 articles

Word: 3165, Table: 5, Figure: 5 Development of a multiplex real-time PCR for the simultaneous detection of monkeypox virus clades I, II and goatpox virus

Provisionally accepted
Yongqiang Lin Yongqiang Lin Zijing Guo Zijing Guo Jinsong Chen Jinsong Chen Xianwen Zhang Xianwen Zhang Long Zhou Long Zhou Yanmin Li Yanmin Li *Zhidong Zhang Zhidong Zhang *
  • College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu, China

The final, formatted version of the article will be published soon.

    Monkeypox virus (MPXV) hosts are of multiple species, with a risk of cross-species transmission.This phenomenon poses a threat to unreported affected domestic animals and increases the risk to human public health. Clinical diagnostics continue to face challenges regarding specificity among poxviruses. The need for a rapid and precise assay to differentiate between MPXV clades I and II, as well as goatpox virus (GTPV) is essential for enhancing our capacity for disease prevention, control, and epidemiological investigation. To address this need, we have successfully developed a multiplex real-time PCR assay targeting MPXV D14L gene for clade I, MPXV D18L gene for clade II, and GTPV RPO30 gene , which can simultaneously detect MPXV clades I and II as well as GTPV. The developed assay demonstrated high sensitivity, with limits of detection at 207.83 copies/reaction for MPXV clade I, 252.07 copies/reaction for MPXV clade II and 208.72 copies/reaction for GTPV.Importantly, there was no cross-reactivity with other non-pox viruses which infect goats. The assay exhibited excellent repeatability, with coefficients of variation (CV%) for intra-assay and inter-assay ranging from 0.17 % to 0.89 % and 0.58 % to 1.09 %, respectively. This assay can serve as a vital resource to safeguard against the MPXV epidemic posing a threat to the life safety of goats, to mitigate potential risks to the sheep farming industry, and to prevent the transmission of MPXV to humans through sheep, which could act as a potential transmission vector for infection.

    Keywords: Monkeypox Virus (MPXV), goat pox virus (GTPV), Clade I, clade II, D14L gene, D18L gene, Multiplex real-time PCR

    Received: 20 Aug 2024; Accepted: 31 Oct 2024.

    Copyright: © 2024 Lin, Guo, Chen, Zhang, Zhou, Li and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yanmin Li, College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu, China
    Zhidong Zhang, College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu, China

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