Skip to main content

ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1480122
This article is part of the Research Topic The Role of Calcium Channels in Human Health and Disease Volume III View all articles

The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients' colorectal cancer and normal colon tissues, and its hepatic effects in rodents

Provisionally accepted
Solomiia Bychkova Solomiia Bychkova 1Mykola Bychkov Mykola Bychkov 2Dani Dordevic Dani Dordevic 3Simon K. Rittmann Simon K. Rittmann 4*Vítězová Monika Vítězová Monika 5Ivan Kushkevych Ivan Kushkevych 5*
  • 1 Ivan Franko National University of Lviv, Lviv, Ukraine
  • 2 Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • 3 University of Veterinary and Pharmaceutical Sciences Brno, Brno, South Moravia, Czechia
  • 4 University of Vienna, Vienna, Austria
  • 5 Masaryk University, Brno, South Moravia, Czechia

The final, formatted version of the article will be published soon.

    Colorectal cancer is influenced by genetic mutations, lifestyle factors, and diet, particularly high fat intake, which raises bile acid levels in the intestinal lumen. This study hypothesized that bile acids contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa.The effects of 3-sulfo-taurolithocholic acid (TLC-S) on ATPase activity were investigated in colorectal cancer samples from 10 patients, using adjacent healthy tissue as controls, and in rodent liver function. ATPase activity was measured spectrophotometrically by determining inorganic phosphorus (P i ) in postmitochondrial fractions. Ca 2+ dynamics were assessed in isolated mouse hepatocytes with fluorescence imaging, and rat liver mitochondria were studied using polarographic methods to evaluate respiration and oxidative phosphorylation. TLC-S increased Na + /K + ATPase activity by 1.5 times in colorectal cancer samples compared to controls (P ≤ 0.05). In healthy mucosa, TLC-S decreased Mg 2+ ATPase activity by 3.6 times (P ≤ 0.05), while Mg 2+ ATPase activity in cancer tissue remained unchanged. TLC-S had no significant effect on Ca 2+ ATPase activity in healthy colon mucosa but showed a trend towards decreased activity in cancer tissue. In rat liver, TLC-S decreased Ca 2+ ATPase and Na + /K + ATPase activities while increasing basal Mg 2+ 1 ATPase activity (P ≤ 0.05). Additionally, TLC-S induced cytosolic Ca 2+ signals in mouse hepatocytes, partially attenuated by NED-19, an NAADP antagonist (P ≤ 0.05). TLC-S also reduced the V3 respiration rate of isolated rat liver mitochondria during α-ketoglutarate oxidation.These findings suggest that TLC-S modulates ATPase activity differently in cancerous and healthy colon tissues, playing a role in colorectal cancer development. In rat liver, TLC-S affects mitochondrial activity and ATPase function, contributing to altered cytosolic calcium levels, providing insight into the mechanistic effects of bile acids on colorectal cancer and liver function.

    Keywords: colorectal cancer, colon mucosae, Na+/K+ ATPase, Ca2+ ATPase, basal Mg2+ ATPase, Bile acid

    Received: 14 Aug 2024; Accepted: 31 Oct 2024.

    Copyright: © 2024 Bychkova, Bychkov, Dordevic, Rittmann, Monika and Kushkevych. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Simon K. Rittmann, University of Vienna, Vienna, Austria
    Ivan Kushkevych, Masaryk University, Brno, 601 77, South Moravia, Czechia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.