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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1477540
This article is part of the Research Topic The application of new technologies such as new vaccines, therapeutic cytokines and antibodies, and antiviral drugs in the prevention and treatment of animal infectious diseases View all 7 articles

Sialoadhesin-Dependent Susceptibility and Replication of Porcine Reproductive and Respiratory Syndrome Viruses in CD163-Expressing Cells

Provisionally accepted
Hyun-Ji Lee Hyun-Ji Lee 1Su-Hwa You Su-Hwa You 1Hyang-Sim Lee Hyang-Sim Lee 1Yeon-Kyung Shin Yeon-Kyung Shin 1Yun Sang Cho Yun Sang Cho 1Tae Sub Park Tae Sub Park 2,3*Seok-Jin Kang Seok-Jin Kang 1*
  • 1 Animal and Plant Quarantine Agency, Gyeongsangbuk, Republic of Korea
  • 2 Graduate School of International Agriculture Technology, Seoul National University, Seoul, Republic of Korea
  • 3 Institute of Greenbio Science Technology, Seoul National University, Seoul, Republic of Korea

The final, formatted version of the article will be published soon.

    Understanding the molecular interactions between porcine reproductive and respiratory syndrome viruses (PRRSVs) and host cells is crucial for developing effective strategies against PRRSV. CD163, predominantly expressed in porcine macrophages and monocytes, is a key receptor for PRRSV infection. CD169, also known as sialoadhesin, has emerged as a potential receptor facilitating PRRSV internalization. In this study, we investigated PRRSV susceptibility in relation to CD169 expression in CD163-expressing cells. Susceptibility to PRRSV infection was estimated by immunostaining the N protein using SR30A and quantifying ORF7 using RT-PCR. PRRSV strains adapted to MARC-145 did not infect CD163+/CD169-cells but successfully replicated in CD163+/CD169+ cells. Similarly, porcine alveolar macrophage-isolated PRRSV strains effectively infected and propagated in CD163+/CD169+ cells compared to CD163+/CD169-cells (100% vs. 82.9%). We confirmed that high CD169 expression in CD163-expressing cells increases susceptibility to PRRSVs compared to low or no CD169 expression. In conclusion, CD169 expression level influences viral entry efficiency into CD163-expressing cells, providing valuable insights for isolating wild PRRSVs and producing high-titer PRRS vaccine candidates.

    Keywords: Porcine Reproductive and Respiratory Syndrome Virus, Sialoadhesin, CD163, Cell Line, Gene Expression

    Received: 08 Aug 2024; Accepted: 22 Nov 2024.

    Copyright: © 2024 Lee, You, Lee, Shin, Cho, Park and Kang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Tae Sub Park, Graduate School of International Agriculture Technology, Seoul National University, Seoul, 08826, Republic of Korea
    Seok-Jin Kang, Animal and Plant Quarantine Agency, Gyeongsangbuk, Republic of Korea

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.