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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1474386
This article is part of the Research Topic Reproductive biotechnologies and challenges in their application View all 10 articles

Supplementation of sperm cooling medium with Eurycoma longifolia extract enhances native Thai chicken sperm quality and fertility potential

Provisionally accepted
Thirawat Koedkanmark Thirawat Koedkanmark 1Ruthaiporn Ratchamak Ruthaiporn Ratchamak 2Supakorn Authaida Supakorn Authaida 1Wuttigrai Boonkum Wuttigrai Boonkum 1Yoswaris Semaming Yoswaris Semaming 3Vibuntita Chankitisakul Vibuntita Chankitisakul 1*
  • 1 Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Mueang, Thailand
  • 2 Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Mahasarakham, Maha Sarakham, Thailand
  • 3 Program in Veterinary Technology, Faculty of Technology, Udon Thani Rajabhat University, Muang, Chiang Mai, Thailand

The final, formatted version of the article will be published soon.

    Cooled semen storage methods result in oxidative stress generated by an imbalance between oxidation rates, specifically reactive oxygen species production, and sperm cell antioxidants, leading to degradation of semen quality. We aimed to investigate the impact of adding Eurycoma longifolia (EL) extract as an antioxidant supplement in semen storage medium (IGGKPh semen extender) on semen quality and fertility potential. EL extract at concentrations of 0, 5, 10, 15, and 20 mg/mL was assessed for its antioxidant capacity in IGGKPh semen extender. Our findings revealed that the total phenolic content in the EL extract did not vary significantly across the various concentrations and temperatures tested. However, incubation at 5°C was found to be the most effective temperature for increasing the EL extract antioxidant capacity as assessed via the 2,2-diphenyl-1-picrylhydrazyl inhibition assay in a dose-dependent manner. Supplementation of the IGGKPh semen extender with 15 mg/mL EL extract was found to enhance semen quality during cold storage for up to 48 hours (P<0.05), as evidenced by decreased malondialdehyde levels in cooled semen (P<0.05). However, antioxidant enzyme activities showed no significant differences among the various experimental groups (P>0.05). The fertility test showed that the 15 mg/mL EL extract group stored for 24 hours had a higher percentage than the control group (P<0.05). However, there was no significant difference in percentage between the two groups at 48 hours of storage (P>0.05). The hatchability showed no significant difference in both 24 and 48hour storage periods (P>0.05). Our results indicated that supplementing the IGGKPh semen extender with 15 mg/mL EL extract may positively influence semen quality during storage, suggesting potential applications for enhancing semen quality.

    Keywords: cold semen preservation, antioxidant, Long Jack, Rooster semen, Lipid peroxiation

    Received: 01 Aug 2024; Accepted: 23 Aug 2024.

    Copyright: © 2024 Koedkanmark, Ratchamak, Authaida, Boonkum, Semaming and Chankitisakul. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Vibuntita Chankitisakul, Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Mueang, Thailand

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.