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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1473408

Establishment of a duplex TaqMan-based real time RT-PCR assay for simultaneous detection of BRSV and BVDV

Provisionally accepted
Fuxing Hao Fuxing Hao 1Jinping Fu Jinping Fu 2Jun Chen Jun Chen 2Daoxian Zhu Daoxian Zhu 1Bingyan Cai Bingyan Cai 1Yuxin Li Yuxin Li 1Chuanmin Liu Chuanmin Liu 2*
  • 1 Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China
  • 2 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Bovine Respiratory Disease Complex (BRDC) represents a global acute respiratory condition that imposes substantial economic burdens on the cattle industry due to its high morbidity and mortality rates. Various factors contribute to the development of BRDC, including pathogen infections, environmental stresses, weaning of calves, and herd relocation. Viral pathogens, notably Bovine Respiratory Syncytial Virus (BRSV) and Bovine Viral Diarrhea Virus (BVDV), play a critical role in the etiology of BRDC, with single or combined viral infections being particularly clinically significant. In this study, we developed a duplex TaqMan-based real-time RT-PCR assay targeting the conserved regions of the F gene of BRSV and the 5'UTR sequence of BVDV. The limits of detection for BRSV and BVDV were 6.83 copies/μL and 5.24 copies/μL respectively. Our validation data suggest the assay has excellent sensitivity, specificity and reproducibility. Testing of clinical samples revealed prevalence of BRSV and BVDV in local farms in Jiangsu Province, China. This study provides an efficient diagnostic tool for the epidemiological investigation of BRDC.

    Keywords: Bovine respiratory disease complex (BRDC), Bovine respiratory syncytial virus (BRSV), Bovine viral diarrhea virus (BVDV), TaqMan, Duplex real-time RT-PCR

    Received: 31 Jul 2024; Accepted: 16 Oct 2024.

    Copyright: © 2024 Hao, Fu, Chen, Zhu, Cai, Li and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chuanmin Liu, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.