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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1470992

Resveratrol and β-hydroxy-β-methylbutyric acid supplementation promotes ileal development and digestive function by altering microbial community abundance and metabolites in Tibetan sheep

Provisionally accepted
Jiacheng Gan Jiacheng Gan Qiurong Ji Qiurong Ji Quyangangmao Su Quyangangmao Su Shengzhen Hou Shengzhen Hou Linsheng Gui Linsheng Gui *
  • Qinghai University, Xining, China

The final, formatted version of the article will be published soon.

    The effects of resveratrol (RES) and β-hydroxy-β-methylbutyric acid (HMB) on phenotype, immunity, digestive enzyme activity and short-chain fatty acids (SCFAs) contents in ileum of Tibetan sheep were investigated. A total of 120 two-month-old Tibetan sheep (15.5 ± 0.14 kg) were randomly allocated to 4 treatments: control group (basal diet), RES group (basal diet + 1.5g RES/d), HMB group (basal diet + 1.25g HMB/d), RES-HMB group (basal diet + 1.5g RES/d + 1.25g HMB/d). Results indicated that dietary RES and (or) HMB supplementation significantly improved the phenotype (mucosal thickness and villus width), SCFAs concentrations, and digestive enzymes (lipase, cellulase, and α-amylase) (P<0.05). The relative abundance of Brevibacillus, Clostridium sensu stricto 3, and Eubacterium hallii group were increased, while the abundance of Ruminococcus and Mogibacterium were decreased (P<0.05) in the RES-HMB group. The metabolic profiling indicated an increase in the differential metabolites (DMs) including L-arginine, butanoic acid, D-mannose, and retinol were increased in the RES-HMB group (P<0.05). In summary, our results suggested that RES and (or) HMB supplementation improved SCFAs concentration by up-regulating the microbial community abundance (Brevibacillus, Clostridium sensu stricto 3, and Eubacterium hallii group) and metabolism (L-arginine, butanoic acid, D-mannose, and retinol), thus contributing to ileal morphology and digestive enzyme activity. These findings may provides a novel reference for the nutritional regulation to improve the production of Tibetan sheep.

    Keywords: resveratrol, β-Hydroxy-β-methylbutyric acid, Tibetan sheep, Ileum, Microorganism, metabolite

    Received: 26 Jul 2024; Accepted: 27 Nov 2024.

    Copyright: © 2024 Gan, Ji, Su, Hou and Gui. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Linsheng Gui, Qinghai University, Xining, China

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