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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Pharmacology and Toxicology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1470781

Effect of caffeic acid grafted chitosan loaded quercetin lyophilized powder formulation on avian colibacillosis and tissue distribution

Provisionally accepted
Xin Ren Xin Ren 1Sikun Yuan Sikun Yuan 2,3Juan Ren Juan Ren 1Leying Ma Leying Ma 1Juxiang Liu Juxiang Liu 1Gengnan Wang Gengnan Wang 1*
  • 1 Hebei Agricultural University, Baoding, China
  • 2 Hebei University, Baoding, Hebei Province, China
  • 3 Baoding Institute for Food and Drug Control, Baoding, Hebei Province, China

The final, formatted version of the article will be published soon.

    Quercetin (QR), recognized as a natural antibacterial ingredient, has found widespread application in the poultry industry. This study investigated the bacteriostatic mechanism and evaluated the in vivo inhibitory impact of caffeic acid-grafted chitosan self-assembled micelles loaded quercetin (CA-g-CS/QR) on avian Escherichia coli (E. coli). The findings indicate that the bactericidal mechanism of CA-g-CS/QR exhibits enhanced efficacy compared to QR alone, disrupting bacterial cell walls, disassembling biofilm structures, and impeding essential components necessary for bacterial growth. Following an avian E. coli attack in broilers, CA-g-CS/QR demonstrated the capacity to enhance the population of beneficial bacteria while concurrently decreasing harmful bacteria within the intestinal tract. Moreover, within three days of oral administration of CA-g-CS/QR, a significant decrease in Escherichia spp. count was evident, resulting in the restoration of broilers to a healthy state. CA-g-CS/QR proved to be a significant and more efficacious solution than QR alone for avian E. coli disease. Furthermore, CA-g-CS/QR displayed a broader distribution range and higher concentration within the body. Ten metabolites have been identified in the liver for both QR and CA-g-CS/QR. In conclusion, CA-g-CS/QR has demonstrated a notable capacity to enhance in vitro and in vivo bacterial inhibitory effects, providing foundation for the clinical application of QR in combating avian E. coli infections in broilers.

    Keywords: Quercetin, Escherichia coli, mechanism of bacterial antibacterial, intestinal flora, Tissue Distribution

    Received: 26 Jul 2024; Accepted: 18 Sep 2024.

    Copyright: © 2024 Ren, Yuan, Ren, Ma, Liu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gengnan Wang, Hebei Agricultural University, Baoding, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.