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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Veterinary Experimental and Diagnostic Pathology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1469586

Colchicine can keep the viability of bacteria in mastitic milk by preventing leukocyte phagocytosis in dairy cow and goat

Provisionally accepted
Keiichi Hisaeda Keiichi Hisaeda 1Masato Hirano Masato Hirano 2Naoki Suzuki Naoki Suzuki 2Naoki Isobe Naoki Isobe 2*
  • 1 Okayama University of Science, Okayama, Japan
  • 2 Hiroshima University, HIgashi-Hiroshima, Japan

The final, formatted version of the article will be published soon.

    Despite the occurrence of mastitis, no bacteria were detected in any of the milk samples after culture. This is partially because the neutrophils present in milk phagocytose bacteria during milk preservation. In this study, we investigated whether colchicine inhibited the decrease in viable bacteria in milk by suppressing phagocytosis during preservation. The number of viable bacteria decreased when cow milk was preserved for 5 h. However, the addition of 0.1 and 1% colchicine significantly increased the number of viable bacteria (P < 0.05). The percentage of culture-negative cow's milk increased more than two-fold after 5 h compared to that at 0 h of preservation, however this percentage was significantly reduced by the addition of colchicine (P < 0.05). When goat milk with mastitis was incubated with bacteria (Escherichia. coli, Klebsiella pneumoniae, and Staphylococcus aureus), the percentage of phagocytosed neutrophils decreased significantly with the addition of colchicine (P < 0.05). These results indicate that colchicine suppressed the decrease in the number of viable bacteria by preventing neutrophil phagocytosis during milk preservation. These findings may help in the identification of mastitis-causing bacteria and the selection of antibiotics for the treatment of mastitis.

    Keywords: Colchicine, Cow, Goat, Milk, Bacteria, Phagocytosis

    Received: 24 Jul 2024; Accepted: 07 Oct 2024.

    Copyright: © 2024 Hisaeda, Hirano, Suzuki and Isobe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Naoki Isobe, Hiroshima University, HIgashi-Hiroshima, Japan

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