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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Livestock Genomics
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1465389
This article is part of the Research Topic Insights in Livestock Genomics View all articles

Integrated Analysis of Muscle LncRNA and mRNA of Chinese Indigenous Breed Ningxiang Pig in Four Developmental Stages

Provisionally accepted
Wenwu Chen Wenwu Chen 1*Fang Yang Fang Yang 1Sui Liufu Sui Liufu 1Zhi Li Zhi Li 2Yan Gong Yan Gong 1Haiming Ma Haiming Ma 1
  • 1 College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
  • 2 Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, Hengyang Normal University, Hengyang 421002, China, Hengyang, China

The final, formatted version of the article will be published soon.

    Meat and its derivatives serve as crucial sources of protein, vitamins, minerals, and other essential nutrients for humans. Pork stands as China's primary animal-derived food product consumed widely across diverse dietary structures; evaluating intramuscular fat content becomes pivotal in assessing its quality standards. Nonetheless, the intricate molecular mechanisms governing intramuscular fat deposition remain elusive. Our study utilized sequencing technology to scrutinize longitudinal development stages within Ningxiang pig's longest dorsal muscles aiming to unravel these underlying mechanisms. In three distinct comparisons (30d vs 90d, 90d vs 150d and 150d vs 210d) there were 578, 1000 and 3238 differentially expressed mRNA, along with 16, 158 and 85 lncRNAs were identified. STEM analysis unveiled six enriched model profiles for lncRNAs while seven such profiles emerged for mRNAs; notably, multiple shared model profiles existed between both RNA types. Enriched analysis highlighted numerous genes from mRNA profile8 and lncRNA profile7 significantly associated with pathways linked to fat deposition. Weight Gene Co-Expression Network Analysis (WGCNA)revealed that differential expression modules (DMEs)& differential expression lncRNAs primarily clustered within cyan, dark slate blue and pale turquoise modules. Furthermore, target genes PKD2 (MSTRG21592.MTRSG8859 and MTRSG18175), COL5A1 (MTRSG9969 and MTRSG180) and SOX13 (MTRSG21592 and MTRSG9088) as core components all intricately tied into processes related to fat deposition. This study lays the groundwork for deeper exploration into the molecular mechanisms underlying LDM fat deposition traits, and it also presents candidate genes for future molecular marker-assisted breeding.

    Keywords: Ningxiang pig1, Muscle2, Transcriptome3, lncRNA4, mRNA5

    Received: 16 Jul 2024; Accepted: 08 Oct 2024.

    Copyright: © 2024 Chen, Yang, Liufu, Li, Gong and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Wenwu Chen, College of Animal Science and Technology, Hunan Agricultural University, Changsha, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.