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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1461116

Construction of recombinant Marek's disease virus co-expressing σB and σC of avian reoviruses

Provisionally accepted
Li Gao Li Gao 1Li Zhong Li Zhong 1Yongzhen Liu Yongzhen Liu 1Changjun Liu Changjun Liu 1Yanping Zhang Yanping Zhang 1Hongyu Cui Hongyu Cui 1Xiaole Qi Xiaole Qi 1Jiayong Zhang Jiayong Zhang 2Jia Xu Jia Xu 2Suyan Wang Suyan Wang 1Yuntong Chen Yuntong Chen 1Yulu Duan Yulu Duan 1Kai Li Kai Li 3*Yulong Gao Yulong Gao 1Xiaomei Wang Xiaomei Wang 1
  • 1 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang Province, China
  • 2 Heilongjiang Provincial Animal Disease Prevention and Control Center, Harbin, Heilongjiang Province, China
  • 3 Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China

The final, formatted version of the article will be published soon.

    Avian reoviruses (ARVs) cause viral arthritis or tenosynovitis, resulting in poor weight gain and increased feed conversion ratios in chickens. In this study, we generated three Marek's disease virus (MDV) recombinants, namely, rMDV-ARV-σB, rMDV-ARV-σC, and rMDV-ARV-σB+C, expressing ARV σB, σC, and both σB and σC, respectively. In rMDV-ARV-σB and rMDV-ARV-σC, the σB or σC gene was inserted into the US2 gene of MDV vaccine strain 814 using a fosmid-based rescue system. In rMDV-ARV-σB+C, the σB and σC genes were cloned into different expression cassettes, which were co-inserted into the US2 gene of the MDV 814 strain. In infected chicken embryo fibroblasts (CEFs), the recombinant virus rMDV-ARV-σB expressed σB, rMDV-ARV-σC expressed σC, and the rMDV-ARV-σB+C virus simultaneously expressed σB and σC. These recombinant viruses exhibited growth kinetics in CEFs similar to those of the parent MDV, and the inserted genes were stably maintained and expressed in the recombinant MDVs after 20 passages in cell cultures. These recombinant MDVs expressing σB and σC will provide potential vaccines against ARV infection in chickens.

    Keywords: Marek's disease virus, Avian reovirus, σB, σC, Vaccine

    Received: 07 Jul 2024; Accepted: 28 Aug 2024.

    Copyright: © 2024 Gao, Zhong, Liu, Liu, Zhang, Cui, Qi, Zhang, Xu, Wang, Chen, Duan, Li, Gao and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Kai Li, Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.