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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1460500

Transcriptome analysis provides new insights into the response of canine intestinal epithelial cells treated by sulforaphane: a natural product of cruciferous origin

Provisionally accepted
Kaiqi Li Kaiqi Li 1Jin Yan Jin Yan 1Shiqi Wang Shiqi Wang 1Chuyang Zhu Chuyang Zhu 1Qi Zhu Qi Zhu 1Sichen Lu Sichen Lu 1Ping Hu Ping Hu 1Tadelle D. Alemayehu Tadelle D. Alemayehu 2In Ho Kim In Ho Kim 3Abdelkareem A. Ahmed Abdelkareem A. Ahmed 4Haoyu Liu Haoyu Liu 1,5Wael Ennab Wael Ennab 1*Demin Cai Demin Cai 1,5*
  • 1 College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China
  • 2 International Livestock Research Institute (Ethiopia), Addis Ababa, Addis Ababa, Ethiopia
  • 3 Department of Animal Resources, Dankook University, Cheonan, South Chungcheong, Republic of Korea
  • 4 Botswana University of Agriculture and Natural Resources, Gabarone, Botswana
  • 5 Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, China, Yangzhou, China

The final, formatted version of the article will be published soon.

    This study presents a comprehensive transcriptome analysis of canine intestinal epithelial cells following treatment with sulforaphane (SFN), a naturally occurring compound found in cruciferous vegetables with established anti-inflammatory and antioxidant properties. Through high-throughput sequencing, we identified 29,993 genes, among which 1,612 were differentially expressed, with 792 up-regulated and 820 down-regulated in response to SFN treatment. Our analysis revealed significant enrichment of genes in pathways associated with the inflammatory response, lipid metabolism, oxidative stress response, and T-cell mediated immunity, suggesting SFN's potential in modulating these biological processes. Notably, the PPARγ gene, which plays a crucial role in the body's oxidative stress and inflammatory response, was highly up-regulated, indicating its possible centrality in SFN's effects. Gene-gene interaction analysis further supported SFN's role in alleviating inflammation through PPARγ, with key genes in oxidative stress and inflammatory response pathways showing significant correlations with PPARγ. Overall, our findings provide molecular evidence for SFN's protective effects on canine intestinal health, potentially through the modulation of inflammatory and oxidative stress pathways, with PPARγ emerging as a critical mediator.

    Keywords: Sulforaphane, transcriptome analysis, canine, intestine, Inflammation response

    Received: 06 Jul 2024; Accepted: 12 Sep 2024.

    Copyright: © 2024 Li, Yan, Wang, Zhu, Zhu, Lu, Hu, Alemayehu, Kim, Ahmed, Liu, Ennab and Cai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wael Ennab, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China
    Demin Cai, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.