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BRIEF RESEARCH REPORT article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 |
doi: 10.3389/fvets.2024.1457719
Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the infection of enteric coronavirus
Provisionally accepted- 1 Hainan Academy of Agricultural Sciences, Haikou, Hainan Province, China
- 2 Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
Intestinal organoids are valuable tools for investigating intestinal physiology and pathology ex vivo. In previous studies, intestinal organoids of commercial pigs have been developed. Here, we established intestinal organoids derived from Wuzhishan miniature pigs (WZS pigs), a unique kind of pig in the Hainan province of China. 3D intestinal organoids and organoids monolayers were developed and assessed. Furthermore, the susceptibility of organoids monolayers of WZS pig to transmissible gastroenteritis virus (TGEV) was demonstrated. RNA-seq analysis revealed that the TGEV infection stimulated antiviral and inflammatory immune responses in organoids monolayers models. The study implied the transmission risk of swine enteric coronavirus on WZS pigs and provided useful tools for further research on WZS pigs as laboratory miniature pig models.
Keywords: intestinal organoids, organoids monolayers, RNA-Seq, TGEV, Wuzhishan pigs
Received: 01 Jul 2024; Accepted: 09 Sep 2024.
Copyright: © 2024 Liu, Yang, Tan, Zhang, Gao, Cai, Zhang, Fu, Liu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yang Li, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
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