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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Comparative and Clinical Medicine
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1446924
This article is part of the Research Topic Translational Advances in Veterinary Medicine: Emerging Trends and Innovations View all articles

The Effect of Morchella esculenta Polysaccharides on the Rectal Microbiota of Mice Challenged with Lipopolysaccharide (LPS)

Provisionally accepted
Yingjun Zhang Yingjun Zhang 1Reng Qiu Reng Qiu 1Zhifeng Zhang Zhifeng Zhang 1MIKHLID ALMUTAIRI MIKHLID ALMUTAIRI 2Shah Nawaz Shah Nawaz 3Shiqi Dong Shiqi Dong 4*
  • 1 Nanyang Normal University, Nanyang, Henan Province, China
  • 2 King Saud University, Riyadh, Riyadh, Saudi Arabia
  • 3 University of Agriculture, Faisalabad, Faisalabad, Punjab, Pakistan
  • 4 Southwest University, Chongqing, China

The final, formatted version of the article will be published soon.

    Intestinal dysfunction is a severe issue in the digestion and absorption of nutrients, while the gut is the most important organ for digesting and absorbing nutrients. We explored the mitigating effect of Morchella esculenta polysaccharides (MEP) on intestinal injury induced by LPS through modulation of intestinal flora. For this purpose, Kunming mice were divided into three groups namely PC, PM and PY. Group PY was treated with MEP, while groups PM and PY were induced by LPS. Results showed that the weight loss of PM was significantly greater than that of the PY group (P < 0.05), and the organ indexes of the lung and spleen in PM group were significantly higher than those in the PC (P < 0.01) and PY (P < 0.05) groups.LPS caused severe injuries in KM mice in group PM, characterized by broken villi; MEP could alleviate this damage in group PY, resulting in relatively intact villi. Serum analysis showed that TNF-ɑ (P < 0.01), IL-6 (P < 0.01), and MDA (P < 0.05) levels were significantly higher in PM group, while IL-10 (P < 0.001), SOD (P < 0.01), and GSH-Px (P < 0.01) were significantly 1 lower in that group. Interestingly, supplementation with MEP could drop TNF-ɑ, IL-10, IL-6, MDA, and increase SOD (P < 0.01) and GSH-Px levels. Gut microbiota analysis obtained 630,323 raw reads and 554,062 clean reads, identifying 3390 ASVs. One phylum and five genera were notably different among animal groups, including Escherichia_Shigella, Limosilactobacillus, unclassified_Geminicoccaceae, unclassified_Rhodobacteraceae, and Parabacteroides (P. distasonis). In conclusion, we found that MEP could mitigate intestinal damages caused by LPS by modulating inflammatory response, oxidative resistance, and the intestinal flora of KM mice. Our results may provide insights for novel treatment options for intestine-related diseases.

    Keywords: Morchella esculenta polysaccharides, LPS, intestinal flora, rectal microbiota, Intestinal dysfunction

    Received: 10 Jun 2024; Accepted: 16 Aug 2024.

    Copyright: © 2024 Zhang, Qiu, Zhang, ALMUTAIRI, Nawaz and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Shiqi Dong, Southwest University, Chongqing, China

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