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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Livestock Genomics
Volume 11 - 2024 |
doi: 10.3389/fvets.2024.1445594
This article is part of the Research Topic Exploring Aquacultural Animals and Plants by Sequencing and Re-Sequencing View all 5 articles
Identification and Expression Profiling of the Bone Morphogenetic Protein Gene Family Based on pearl culture in mantle and visceral mass of Hyriopsis cumingii
Provisionally accepted- 1 Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
- 2 Shanghai Mugao Biotechnology Co., Lt'd, Shanghai, China
- 3 Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China
- 4 Department of Pediatrics, Shanghai Sixth People's Hospital, Shanghai, China
Bone morphogenetic proteins (BMPs) play an important biological role in pearl biomineralization in pearl mussels. In this study, based on the genome data of the triangular sail mussel (Hyriopsis cumingii), the genome-wide identification and bioinformatic analysis of BMP gene family were performed, and the expression pattern of the BMP genes was investigated by the insertion experiments. The results showed that a total of 12 BMP gene family members (BMP2a/2b, BMP3, BMP5a/5b, BMP7a/7b/7c, BMP9, BMP10a/10b and BMP11) were identified, which were unevenly distributed on chromosome 3/14/18, encoding 169-583 amino acids, with molecular weights ranging from 19.32 to 65.99 kDa. BMP2a, BMP7b and BMP10a were distributed respectively in the cytoplasm, endoplasmic reticulum and mitochondria, others were distributed in the nucleus. qRT-PCR results showed the significant tissue specificity in BMPs gene expression. The HcBMPs were differentially expressed in the mantle and visceral mass, and the expression level was higher in the visceral mass. The expressing trends of HcBMPs were not consistent between the mantle and visceral mass insertion, suggesting that HcBMPs may perform different functions. We also found that insertion surgery in the mantle and visceral mass significantly alters the expression profiling of the BMP gene family. Insertion of the mantle induced the biomineralization function of BMP2a, BMP7a and BMP7b, while BMP3 and BMP10b played opposite roles in visceral mass insertion. Visceral mass insertion could suppress BMP9 expression at 5 d and BMP5b expression at 90 d after insertion This work lays the foundation and data support for the preliminary elucidation of regulatory role and mechanism of HcBMPs in the pearlcultivating process of mantle and visceral mass.
Keywords: Hyriopsis cumingii, Bone Morphogenetic Proteins, IDENTIFICATION, expression analysis, Pearl culture
Received: 17 Jun 2024; Accepted: 29 Jul 2024.
Copyright: © 2024 Chen, Liu, Yao, Sun, Chen, Yu, Xuan, Bian and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Shijun Liu, Shanghai Mugao Biotechnology Co., Lt'd, Shanghai, China
Yuanbin Yao, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
Junlin Sun, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
Xiaofeng Chen, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
Xiangyang Yu, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
Xingrong Xuan, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
Xiangli Bian, Department of Pediatrics, Shanghai Sixth People's Hospital, Shanghai, China
Wenjuan Li, Key Laboratory of Freshwater Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
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