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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Parasitology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1444741
This article is part of the Research Topic The Scourge of Zoonotic and Veterinary Important Tapeworms View all articles

Evaluation of protective immune responses induced by DNA vaccines encoding Echinococcus granulosus EgM123 protein in Beagle dogs

Provisionally accepted
Zhengrong Wang Zhengrong Wang 1,2*Ning Wang Ning Wang 2Jinwen Xian Jinwen Xian 2Pengpeng Zhao Pengpeng Zhao 2Wenqing Zhao Wenqing Zhao 2Na Pu Na Pu 2Xinyue Jia Xinyue Jia 2Yanyan Zhang Yanyan Zhang 2Xinwen Bo Xinwen Bo 2
  • 1 Xinjiang Academy of Agricultural and Reclamation Sciences (XAARS), Shihezi, China
  • 2 Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang Uyghur Region, China

The final, formatted version of the article will be published soon.

    Echinococcus granulosus, known as cystic echinococcosis, is a prominent zoonotic parasitic disease of significant global concern. The definitive hosts serves as the primary reservoir for the transmission of echinococcosis, as well as a main factor in the prevention and control of the disease. Unfortunately, there is currently no commercially available vaccine for these hosts. Nevertheless, DNA vaccines show potential as a feasible strategy for the control and management of parasitic diseases. In this study, the EgM123 antigen was selected for its well-documented immunogenic properties to develop a DNA vaccine aimed at combating E. granulosus infection in canines. The results showed a marked increase in IgG levels in the group vaccinated with pVAX1-EgM123 DNA compared to the PBS group. Additionally, the cytokines IL-1, IFN-γ, IL-4, and IL-6 were significantly upregulated in the pVAX1-EgM123 DNA vaccine group. Furthermore, in comparison to the PBS control group, the EgM123 DNA vaccine group exhibited a notable 87.85% reduction in worm burden and a 65.00% inhibition in segment development. These findings indicate that the pVAX1-EgM123 DNA vaccine shows promising immunogenicity, successfully eliciting a targeted immune response in canines.Moreover, it significantly diminishes the worm burden and hinders the progression of tapeworms in the pVAX1-EgM123 DNA vaccine group. These findings suggest that the pVAX1-EgM123 DNA vaccine holds promise as a potential candidate vaccine for combating E. granulosus infection in dogs.

    Keywords: Echinococcus granulosus, EgM123, DNA vaccine, definitive-host, Worm burden

    Received: 06 Jun 2024; Accepted: 09 Sep 2024.

    Copyright: © 2024 Wang, Wang, Xian, Zhao, Zhao, Pu, Jia, Zhang and Bo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhengrong Wang, Xinjiang Academy of Agricultural and Reclamation Sciences (XAARS), Shihezi, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.