AUTHOR=Sirivisoot Sirintra , Kasantikul Tanit , Techangamsuwan Somporn , Rungsipipat Anudep 

TITLE=Single nucleotide polymorphism profiles of canine T-cell and null-cell lymphomas

JOURNAL=Frontiers in Veterinary Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1439706

DOI=10.3389/fvets.2024.1439706

ISSN=2297-1769

ABSTRACT=<sec id="sec1"><title>Background</title><p>The histopathological classification of T-cell lymphoma (TCL) in humans has distinctive mutational genotyping that suggests different lymphomagenesis. A similar concept is assumed to be observed in dogs with different TCL phenotypes.</p></sec><sec id="sec2"><title>Objective</title><p>This study aimed to identify the previously reported single-nucleotide polymorphisms (SNPs) in both human beings and dogs in canine TCLs and null-cell lymphomas (NCLs) and to design compatible oligonucleotides from each variant based on the multiplex polymerase chain reaction.</p></sec><sec id="sec3"><title>Methods</title><p>Genomic DNA was extracted from 68 tumor specimens (62 TCLs and 6 NCLs) and 5 buffy coat samples from dogs with TCL. Four TCL subtypes and NCL were analyzed in 44 SNPs from 21 genes using the MassARRAY.</p></sec><sec id="sec4"><title>Results</title><p>The greatest incidences of SNPs observed in all TCL subtypes and NCL ware <italic>SATB1</italic> c.1259A &gt; C, <italic>KIT</italic> c.1275A &gt; G, <italic>SEL1L</italic> c.2040 + 200C &gt; G, and <italic>TP53</italic> c.1024C &gt; T, respectively. Some SNP locations were statistically significant associated with NCL, including <italic>MYC</italic> p.S75F (<italic>p</italic> = 0.0003), <italic>TP53</italic> p.I149N (<italic>p</italic> = 0.030), <italic>PDCD1</italic> p.F37LX (<italic>p</italic> = 0.012), and <italic>POT1</italic> p.R583* (<italic>p</italic> = 0.012).</p></sec><sec id="sec5"><title>Conclusion</title><p>Each TCL histological subtype and NCL are likely to contain distinctive mutational genetic profiles, which might play a role in lymphoma gene-risk factors and might be useful for selecting therapeutic target drugs for each canine patient.</p></sec>