AUTHOR=Sabukunze Serges , Gu Haorong , Zhao Lin , Jia Honglin , Guo Huanping 

TITLE=Comparison of the performance of SAG2, GRA6, and GRA7 for serological diagnosis of Toxoplasma gondii infection in cats

JOURNAL=Frontiers in Veterinary Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1423581

DOI=10.3389/fvets.2024.1423581

ISSN=2297-1769

ABSTRACT=<p>Toxoplasmosis is an important zoonotic disease caused by <italic>Toxoplasma gondii</italic> that can infect almost all warm-blooded animals worldwide, including humans. The high prevalence of <italic>T. gondii</italic> infection and its ability to cause serious harm to humans and animals, especially immunodeficient individuals, make it a key public health issue. Accurate diagnostic tools with high sensitivity are needed for controlling <italic>T. gondii</italic> infection. In the current study, we compared the performance of recombinant SAG2, GRA6, and GRA7 in ELISA for the serological diagnosis of <italic>T. gondii</italic> infection in cats. We further investigated the antigenicity of recombinant dense granule protein 3 (rGRA3), rGRA5, rGRA8, and rSRS29A expressed in a plant-based, cell-free expression system for detecting antibodies in <italic>T. gondii</italic>-infected cats. In summary, our data suggest that GRA7 is more sensitive than the other two antigens for the serodiagnosis of <italic>T. gondii</italic> infection in cats, and GRA3 expressed in the cell-free system is also a priming antigen in serological tests for detecting <italic>T. gondii</italic> infection in cats.</p>