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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1423122
This article is part of the Research Topic Infectious Diseases Associated with Reproductive Disorders in Domestic Animals View all 9 articles

Isolation and Identification of a Novel Goose-Origin Reovirus GD218 and Its Pathogenicity Experiments

Provisionally accepted
Yuze Li Yuze Li Huihu Yang Huihu Yang Yongkun Lu Yongkun Lu Zhenghao Yin Zhenghao Yin Hang Xu Hang Xu Kun Mei Kun Mei *Shuanjian Huang Shuanjian Huang
  • Foshan University, Foshan, China

The final, formatted version of the article will be published soon.

    Since 2020, a novel goose-derived reovirus, characterized by goose hemorrhagic hepatitis, has emerged in the goose breeding industry of Guangdong Province, China, leading to significant economic losses in the poultry sector. To study the genetic variation of novel goose reovirus (NGRV) in Guangdong Province, this experiment utilized goose embryonic fibroblast cells for virus isolation. RT-PCR was conducted to identify, amplify, clone, and sequence the complete genome of the NGRV isolated from Zhaoqing. The genomic sequences were compared with reference strains to construct a phylogenetic tree. Moreover, animal pathogenicity, excretion patterns, and pathological sections were examined. The results showed that liver and spleen samples from geese suspected of NGRV infection were used for isolation, resulting in the identification of a reovirus presumed to originate from geese, designated as GD218. In terms of genomic structure and sequence homology, GD218 closely resembles the novel duck reovirus, differing significantly from earlier isolated NDRV strains (J18, NP03, SD12, etc.) in genetic composition (nt: 80.6~97.9%, aa: 94.3~98.9%). However, it is similar to strains isolated after 2018, such as XT18, SY, QR, YL, LY20, etc. (nt: 95.3~98.9%, aa: 98.6~99.7%). Therefore, based on phylogenetic analysis, GD218 is hypothesized to be a novel type of goose-origin reovirus homologous to the novel duck reovirus.

    Keywords: Novel goose reovirus, SEPARATION, whole genome sequencing, genetic analysis, pathogenicity

    Received: 25 Apr 2024; Accepted: 10 Sep 2024.

    Copyright: © 2024 Li, Yang, Lu, Yin, Xu, Mei and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Kun Mei, Foshan University, Foshan, China

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