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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 11 - 2024 |
doi: 10.3389/fvets.2024.1420164
Whole-genome sequencing identified candidate genes associated with high and low litter size in Chuanzhong black goats
Provisionally accepted
Conghui Guo
Junning Ye
Jie Liu
Zhihan Li
Ming Deng
Yongqing Guo
Guangbin Liu
Baoli Sun
Yaokun Li
*
Dewu Liu
*- South China Agricultural University, Guangzhou, China
The reproductive performance of goats significantly influences breeding efficiency and economic returns, with litter size serving as a comprehensive indicator. Despite this, research on the genetic control of litter size remains limited. Therefore, we aimed to explore the candidate genes affecting fecundity and compared the whole-genome sequences (WGS) of 15 high-litter (HL) and 15 low-litter (LL) size in Chuanzhong black goats. Then genetic diversity and genomic variation patterns were analyzed by phylogenetic, principal component and population genetic structure analysis, it was found that HL and LL subpopulations diverged. Population evolutionary selection elimination analysis was performed by Fst and θπ resulted in 506 genes were annotated in HL and 528 genes in LL. These genes were mainly related to Hippo signaling pathway, G protein-coupled signaling pathway, G protein-coupled receptor activity, cell surface receptor signaling pathway, gonadal and reproductive structure development. According to the significantly selected genomic regions and important pathways, we found that the g.89172108T > G variant locus in the exon of the AMH gene was significantly associated with litter size (P < 0.05), which could be used as an auxiliary selection gene for the high fertility of Chuanzhong black Goat.
Keywords: Litter Size, whole genome sequencing, Chuanzhong black goats, AMH, Reproduction
Received: 19 Apr 2024; Accepted: 09 Sep 2024.
Copyright: © 2024 Guo, Ye, Liu, Li, Deng, Guo, Liu, Sun, Li and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yaokun Li, South China Agricultural University, Guangzhou, China
Dewu Liu, South China Agricultural University, Guangzhou, China
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