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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1417590

Comparative Transcriptome Analysis of Whiteflies raised on Cotton leaf Curl Multan virus infected cotton plants

Provisionally accepted
Ting Chen Ting Chen *yanbo Jia yanbo Jia *Jie Chen Jie Chen *Guojun QI Guojun QI *
  • Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China

The final, formatted version of the article will be published soon.

    Cotton leaf curl Multan virus (CLCuMuV), a serious viral disease-causative agent in cotton plants in South Asia, is transmitted by Bemisia tabaci cryptic species complex in a persistent-circulative manner. Previous study indicated that Asia II-7 whiteflies could transmit CLCuMuV while Mediterranean (MED) failed to. However, little is known about the genes involved in this process. In this study, Asia II-7 and MED B.tabaci were utilized to determine transcriptomic responses after 48h acquisition access periods (AAPs). As a result, 14,213 and 8,986 differentially expressed genes (DEGs) were identified. Furthermore, DEGs related to the immune system and metabolism of Asia II-7 and MED in response to CLCuMuV-infected plants were identified and analyzed using Gene Ontologies (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the number of related DEGs in MED was lower than that of Asia II-7. The most abundant groups of DEGs between both viruliferous and aviruliferous whitefly species were zf-C2H2 family of transcription factors (TFs). Notably, in comparison to viruliferous MED, Asia II-7 exhibited more DEGs related to cathepsin biosynthesis. Overall, this study provides the basic information for investigating the molecular mechanism of how begomoviruses affect B.tabaci metabolism and immune response either as vector cryptic species or non-vector species.

    Keywords: Cotton leaf curl Multan virus 1, Bemisia tabaci 2, cryptic species 3, transcriptome 4, expression profiles 5

    Received: 17 Apr 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Chen, Jia, Chen and QI. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ting Chen, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
    yanbo Jia, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
    Jie Chen, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
    Guojun QI, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China

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