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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1415685
This article is part of the Research Topic Bacteriophages, a weapon against animal bacterial pathogens and biofilms View all articles

Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages

Provisionally accepted
Yuzhi Wang Yuzhi Wang 1Guixiang Tong Guixiang Tong 2*Xinglong Jiang Xinglong Jiang 3*Chuandeng Tu Chuandeng Tu 4Hongjiao Cai Hongjiao Cai 1*Wenhong Fang Wenhong Fang 5*Honglian Tan Honglian Tan 2*Qibiao Weng Qibiao Weng 6*Xinxian Wei Xinxian Wei 2*Mao Lin Mao Lin 1,3*
  • 1 State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian Province, China
  • 2 Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi Zhuang Region, China
  • 3 Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Fisheries College of Jimei University, Xiamen, Fujian, China
  • 4 Xiamen Ocean Vocational College, Xiamen, Fujian Province, China
  • 5 East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China
  • 6 Key Laboratory of Eel Aquaculture and Processing of Fujian Province, Fuzhou, China

The final, formatted version of the article will be published soon.

    In this study, a novel virulent Aeromonas hydrophila phage designated phiA051 has been isolated from aquaculture water and identified from biological and genomic perspective. Electron microscopic observation showed that the phage phiA051 was composed of an icosahedral capsid with an isometric diameter of 71.3±2.0 nm, and a tail with length of 73.1±2.7 nm. The phage phiA051 possesses an optimal multiplicity of infection (MOI) of 0.01, and its burst size was 108 PFU/cell. The phage maintained a high viability at temperatures of 30-50℃ or pH 6.0-10.0 for 1 h. Inhibitory capacity against A051 showed that the phage phiA051 has certain potentials in rapidly inhibiting the spread of pathogen early in the outbreak. The whole-genome sequencing revealed that phage phiA051 had a linear dsDNA with GC content of 60.55% and a total length of 32,212 bp, including 46 ORFs. The phage phiA051 behaved as a virulent phage. However, the BLASTN result showed that 23 of the top 25 hits were genomes of Aeromonas strains. It was suggested that phiA051 was probably derived from some prophage in the chromosome of Aeromonas. Further investigation

    Keywords: Aeromonas hydrophila1, bacteriophage2, biological characteristics3, whole genome4, prophage5

    Received: 11 Apr 2024; Accepted: 05 Jul 2024.

    Copyright: © 2024 Wang, Tong, Jiang, Tu, Cai, Fang, Tan, Weng, Wei and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Guixiang Tong, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, Guangxi Zhuang Region, China
    Xinglong Jiang, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Fisheries College of Jimei University, Xiamen, 361021, Fujian, China
    Hongjiao Cai, State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian Province, China
    Wenhong Fang, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China
    Honglian Tan, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, Guangxi Zhuang Region, China
    Qibiao Weng, Key Laboratory of Eel Aquaculture and Processing of Fujian Province, Fuzhou, China
    Xinxian Wei, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, Guangxi Zhuang Region, China
    Mao Lin, State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian Province, China

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