AUTHOR=Manyenya Sophina , Nthiwa Daniel , Lutta Harrison Osundwa , Muturi Mathew , Nyamota Richard , Mwatondo Athman , Watene Grace , Akoko James , Bett Bernard 

TITLE=Multiple pathogens co-exposure and associated risk factors among cattle reared in a wildlife-livestock interface area in Kenya

JOURNAL=Frontiers in Veterinary Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1415423

DOI=10.3389/fvets.2024.1415423

ISSN=2297-1769

ABSTRACT=<sec id="sec1"><title>Introduction</title><p>Understanding multi-pathogen infections/exposures in livestock is critical to inform prevention and control measures against infectious diseases. We investigated the co-exposure of foot-and-mouth disease virus (FMDV), <italic>Brucella</italic> spp., <italic>Leptospira</italic> spp., and <italic>Coxiella burnetii</italic> in cattle in three zones stratified by land use change and with different wildlife-livestock interactions in Narok county, Kenya. We also assessed potential risk factors associated with the transmission of these pathogens in cattle.</p></sec><sec id="sec2"><title>Methods</title><p>We identified five villages purposively, two each for areas with intensive (zone 1) and moderate wildlife-livestock interactions (zone 2) and one for locations with low wildlife-livestock interactions (zone 3). We sampled 1,170 cattle from 390 herds through a cross-sectional study and tested the serum samples for antibodies against the focal pathogens using enzyme-linked immunosorbent assay (ELISA) kits. A questionnaire was administered to gather epidemiological data on the putative risk factors associated with cattle’s exposure to the investigated pathogens. Data were analyzed using the Bayesian hierarchical models with herd number as a random effect to adjust for the within-herd clustering of the various co-exposures among cattle.</p></sec><sec id="sec3"><title>Results</title><p>Overall, 88.0% (95% CI: 85.0–90.5) of the cattle tested positive for at least one of the targeted pathogens, while 41.7% (95% CI: 37.7–45.8) were seropositive to at least two pathogens. FMDV and <italic>Brucella</italic> spp. had the highest co-exposure at 33.7% (95% CI: 30.9–36.5), followed by FMDV and <italic>Leptospira</italic> spp. (21.8%, 95% CI: 19.5–24.4), <italic>Leptospira</italic> spp. and <italic>Brucella</italic> spp. (8.8%, 95% CI: 7.2–10.6), FMDV and <italic>C. burnetii</italic> (1.5%, 95% CI: 0.7–2.8), <italic>Brucella</italic> spp. and <italic>C. burnetii</italic> (1.0%, 95% CI: 0.3–2.2), and lowest for <italic>Leptospira</italic> spp. and <italic>C. burnetii</italic> (0.3%, 95% CI: 0.0–1.2). Cattle with FMDV and <italic>Brucella</italic> spp., and <italic>Brucella</italic> spp. and <italic>Leptospira</italic> spp. co-exposures and those simultaneously exposed to FMDV, <italic>Brucella</italic> spp. and <italic>Leptospira</italic> spp. were significantly higher in zone 1 than in zones 2 and 3. However, FMDV and <italic>Leptospira</italic> spp. co-exposure was higher in zones 1 and 2 than zone 3.</p></sec><sec id="sec4"><title>Discussion/conclusion</title><p>We recommend the establishment of a One Health surveillance system in the study area to reduce the morbidity of the targeted zoonotic pathogens in cattle and the risks of transmission to humans.</p></sec>