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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1411520

Assessment of the potential risks in SD rats gavaged with genetically modified yeast containing the cp4-epsps gene

Provisionally accepted
Hui Zhao Hui Zhao 1*Bo Bi Bo Bi 1Xuewei Fu Xuewei Fu 1Xuewen Jian Xuewen Jian 1Yu Zhang Yu Zhang 1Yizhi Jiang Yizhi Jiang 2Wuyi Zhou Wuyi Zhou 1
  • 1 South China Agricultural University, Guangzhou, China
  • 2 Zhixin High School, Guangzhou, China

The final, formatted version of the article will be published soon.

    This study evaluates the potential risk of CP4-EPSPS protein in genetically modified (GM) crops. The expression level of CP4-EPSPS in recombinant yeast was quantified to establish daily dosage for gavage. A 90-day feeding experiment on Sprague-Dawley (SD) rats was conducted, with subsequent metabolomics and metagenomic analysis. The results from the physiological and biochemical analysis, organ pathological, blood metabolism, gut microbiota, and correlation analysis of metabolites and gut microbiota revealed several biomarkers for further risk assessment. These include clinical biochemical indexes such as total bilirubin (TBIL), direct bilirubin (DBIL), creatine kinase (CK), and lactate dehydrogenase (LDH); metabolites like Methionine, 2-Oxovaleric acid, and LysoPC (16:0); and gut microbiota including Blautia wexlerae, Holdemanella biformis, Dorea sp CAG 317, Coriobacteriaceae and Erysipelotrichaceae.This study also found that the risk associated with activated recombinant CP4-EPSPS can be substantially mitigated through inactivation. Thus, in routine life, the risk associated with consuming GM foods containing recombinant CP4-EPSPS is substantially reduced after heat treatment.

    Keywords: CP4-EPSPS protein, biomarkers, Metabonomics, gut microbiome, Food Safety

    Received: 03 Apr 2024; Accepted: 21 Jun 2024.

    Copyright: © 2024 Zhao, Bi, Fu, Jian, Zhang, Jiang and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hui Zhao, South China Agricultural University, Guangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.