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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Neurology and Neurosurgery
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1404770

In situ assessment of neuroinflammatory cytokines in different stages of ovine natural prion disease

Provisionally accepted
MARTA MONZON MARTA MONZON *ISABEL M. GUIJARRO ISABEL M. GUIJARRO MOISÈS GARCÉS MOISÈS GARCÉS JUAN J. BADIOLA JUAN J. BADIOLA
  • University of Zaragoza, Zaragoza, Spain

The final, formatted version of the article will be published soon.

    According to the neuroinflammatory hypothesis, a cytokine-mediated host innate immune response may be involved in the mechanisms that contribute to the process of neurodegeneration. Specifically, regarding prion diseases, some experimental murine models have evidenced an altered profile of inflammatory intermediaries. However, the local inflammatory response has rarely been assessed, and never in tissues from different natural models throughout the progression of neurodegeneration. The aim of this study was to use immunohistochemistry (IHC) to in situ assess the temporal protein expression of several cytokines in the cerebellum of sheep suffering from various clinical stages of scrapie. Clear changes in the expression of most of the assessed markers were observed in the affected sheep compared to the healthy control sheep, and from different stages. In summary, this preliminary IHC study focusing in the Purkinje cell layer changes demonstrate that all cytokines or respective receptors studied (IL-1, IL-1R, IL-2R, IL-6, IL-10R and TNFαR) except for IFNγR are disease-associated signaling proteins showing an increase or decrease in relation to the progression of clinical disease. In the future, this study will be extended to other inflammatory mediators and brain regions, focusing in particular on the release of these inflammatory mediators by astroglial and microglial populations.

    Keywords: cytokine, Neuroinflammation, Scrapie, Prion Disease, progress of neurodegenerative disorders, Purkinje Cells

    Received: 04 Apr 2024; Accepted: 18 Sep 2024.

    Copyright: © 2024 MONZON, GUIJARRO, GARCÉS and BADIOLA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: MARTA MONZON, University of Zaragoza, Zaragoza, Spain

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