AUTHOR=Liu Yunjiang , Wang Jiali , Zheng Haohong , Xin Jialiang , Zhong Zhijun , Liu Haifeng , Fu Hualin , Zhou Ziyao , Qiu Xianmeng , Peng Guangneng 

TITLE=Multi-functional properties of lactic acid bacteria strains derived from canine feces

JOURNAL=Frontiers in Veterinary Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1404580

DOI=10.3389/fvets.2024.1404580

ISSN=2297-1769

ABSTRACT=<sec id="sec1"><title>Introduction</title><p>Probiotics, especially Lactic Acid Bacteria (LAB), can promote the health of host animals in a variety of ways, such as regulating intestinal flora and stimulating the host’s immune system.</p></sec><sec id="sec2"><title>Methods</title><p>In this study, 206 LAB strains were isolated from 48 canine fecal samples. Eleven LAB strains were selected based on growth performance, acid and bile salt resistance. The 11 candidates underwent comprehensive evaluation for probiotic properties, including antipathogenic activity, adhesion, safety, antioxidant capacity, and metabolites.</p></sec><sec id="sec3"><title>Results</title><p>The results of the antipathogenic activity tests showed that 11 LAB strains exhibited strong inhibitory effect and co-aggregation ability against four target pathogens (<italic>E. coli</italic>, <italic>Staphylococcus aureus</italic>, <italic>Salmonella braenderup</italic>, and <italic>Pseudomonas aeruginosa</italic>). The results of the adhesion test showed that the 11 LAB strains had high cell surface hydrophobicity, self-aggregation ability, biofilm-forming ability and adhesion ability to the Caco-2 cells. Among them, <italic>Lactobacillus acidophilus</italic> (L177) showed strong activity in various adhesion experiments. Safety tests showed that 11 LAB strains are sensitive to most antibiotics, with L102, L171, and L177 having the highest sensitivity rate at 85.71%, and no hemolysis occurred in all strains. Antioxidant test results showed that all strains showed good H<sub>2</sub>O<sub>2</sub> tolerance, high scavenging capacity for 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) and hydroxyl (OH<sup>−</sup>). In addition, 11 LAB strains can produce high levels of metabolites including exopolysaccharide (EPS), γ-aminobutyric acid (GABA), and bile salt hydrolase (BSH).</p></sec><sec id="sec4"><title>Discussion</title><p>This study provides a thorough characterization of canine-derived LAB strains, highlighting their multifunctional potential as probiotics. The diverse capabilities of the strains make them promising candidates for canine dietary supplements, offering a holistic approach to canine health. Further research should validate their efficacy <italic>in vivo</italic> to ensure their practical application.</p></sec>