AUTHOR=Andrei Crina Raluca , Posastiuc Florin Petrișor , Constantin Nicolae Tiberiu , Mitrea Ioan Liviu 

TITLE=New insights into semen separation techniques in buffaloes

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1347482

DOI=10.3389/fvets.2023.1347482

ISSN=2297-1769

ABSTRACT=<p>Male infertility is frequently caused by idiopathic or unexplained reasons, resulting in an increase in demand for assisted reproductive technologies. In buffaloes, more than in other animals due to reproductive hardiness, successful fertilization needs spermatozoa to effectively transit the female reproductive system to reach the oocyte. This mechanism naturally picks high-quality sperm cells for conception, but when artificial reproductive technologies such as <italic>in vitro</italic> fertilization, intracytoplasmic sperm injection, or intrauterine insemination are utilized, alternative techniques of sperm selection are necessary. Currently, technology allows for sperm sorting based on motility, maturity, the lack of apoptotic components, proper morphology, and even sex. This study provides current knowledge on all known techniques of sperm cell sorting in buffaloes, evaluates their efficiency, and discusses the benefits and drawbacks of each approach.</p>