AUTHOR=Zong Bingbing , Xiao Yong , Li Rui , Li Huanhuan , Wang Peiyi , Yang Xiaopei , Zhang Yanyan 

TITLE=Transcriptome and metabolome profiling to elucidate the mechanism underlying the poor growth of Streptococcus suis serotype 2 after orphan response regulator CovR deletion

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1280161

DOI=10.3389/fvets.2023.1280161

ISSN=2297-1769

ABSTRACT=<p>The deletion of orphan response regulator CovR reduces the growth rate of <italic>Streptococcus suis</italic> serotype 2 (<italic>S. suis</italic> 2). In this study, metabolome and transcriptome profiling were performed to study the mechanisms underlying the poor growth of <italic>S. suis</italic> 2 caused by the deletion of orphan response regulator CovR. By comparing <italic>S. suis</italic> 2 (Δ<italic>covR</italic>) and <italic>S. suis</italic> 2 (SC19), 146 differentially accumulated metabolites (upregulated: 83 and downregulated: 63) and 141 differentially expressed genes (upregulated: 86 and downregulated: 55) were identified. Metabolome and functional annotation analysis revealed that the growth of Δ<italic>covR</italic> was inhibited by the imbalance aminoacyl tRNA biosynthesis (the low contents of L-lysine, L-aspartic acid, L-glutamine, and L-glutamic acid, and the high content of L-methionine). These results provide a new insight into the underlying poor growth of <italic>S. suis</italic> 2 caused by the deletion of orphan response regulator CovR. Metabolites and candidate genes regulated by the orphan response regulator CovR and involved in the growth of <italic>S. suis</italic> 2 were reported in this study.</p>