AUTHOR=Onishi-Sakamoto Saki , Fujii Tadashi , Watanabe Keito , Makida Reina , Iyori Keita , Toyoda Yoichi , Tochio Takumi , Nishifuji Koji 

TITLE=Erythritol alters phosphotransferase gene expression and inhibits the in vitro growth of Staphylococcus coagulans isolated from canines with pyoderma

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1272595

DOI=10.3389/fvets.2023.1272595

ISSN=2297-1769

ABSTRACT=<p><italic>Staphylococcus coagulans</italic> (SC) belongs to a group of coagulase-positive staphylococci occasionally isolated from the skin lesions of dogs with pyoderma. We recently revealed that erythritol, a sugar alcohol, inhibited the growth of SC strain JCM7470. This study investigated the molecular mechanisms involved in this growth inhibition of JCM7470 by erythritol, and determine whether erythritol inhibits the growth of SC isolated from the skin of dogs with pyoderma. Comprehensive analysis of the gene expression of JCM7470 in the presence of erythritol revealed that erythritol upregulated the expression of <italic>glcB</italic> and <italic>ptsG</italic> genes, both of which encode phosphotransferase system (PTS) glucoside- and glucose-specific permease C, B, and A domains (EIICBA), respectively, associated with sugar uptake. Moreover, erythritol suppressed <italic>in vitro</italic> growth of all 27 SC strains isolated from the skin lesions of canine pyoderma, including 13 <italic>mecA</italic> gene-positive and 14 <italic>mecA</italic> gene-negative strains. Finally, the growth inhibition of the SC clinical isolates by erythritol was restored by the addition of glucose. In summary, we revealed that erythritol promotes PTS gene expression and suppresses the <italic>in vitro</italic> growth of SC clinical isolates from dogs with pyoderma. Restoration of the erythritol-induced growth inhibition by glucose suggested that glucose starvation may contribute to the growth inhibition of SC.</p>