AUTHOR=Casa Mariana da Silva , Giovambattista Guillermo , Fonteque Graziela Vieira , Miguel Ellen Lara , Vogel Carla Ivane Ganz , Miletti Luiz Claudio , Takeshima Shin-Nosuke , Fonteque Joandes Henrique 

TITLE=Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1256928

DOI=10.3389/fvets.2023.1256928

ISSN=2297-1769

ABSTRACT=<sec><title>Introduction</title><p>The BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis.</p></sec><sec><title>Methods</title><p>This study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of <italic>Anaplasma marginale</italic>, <italic>Babesia bovis</italic>, and <italic>Babesia bigemina</italic> infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association.</p></sec><sec><title>Results</title><p>Of the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to <italic>A. marginale</italic> infections: <italic>BoLA-DRB3001:01</italic> (<italic>p</italic> &lt; 0.001; OR = 0.224), which had a frequency of 7.93%, and <italic>BoLA-DRB3024:06</italic> (<italic>p</italic> = 0.007; OR &lt; 0.00001), which had a frequency of 0.72%. Regarding <italic>B. bovis</italic> infection, the <italic>BoLA-DRB3*011:01</italic> allele (<italic>p</italic> = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by <italic>B. bigemina</italic>.</p></sec><sec><title>Discussion</title><p>The Crioulo Lageano breed has alleles that may confer resistance against infection by <italic>A. marginale</italic> and <italic>B. bovis</italic>.</p></sec>