AUTHOR=Kim Dong-Ju , Kim Yong-Chan , Jeong Byung-Hoon 

TITLE=First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1229369

DOI=10.3389/fvets.2023.1229369

ISSN=2297-1769

ABSTRACT=<p>Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (<italic>PRNP</italic>) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, and genetic variations of the leporine <italic>PRNP</italic> gene have not been investigated to date. To identify leporine <italic>PRNP</italic> gene polymorphism, we performed amplicon sequencing in 203 rabbits. We report a novel single nucleotide polymorphism on the leporine <italic>PRNP</italic> gene. In addition, we performed a comparative analysis of amino acid sequences of prion protein (PrP) across several hosts using ClustalW2. Furthermore, we evaluated the effect of changes of unique leporine PrP amino acids with those conserved among various species using Swiss-Pdb Viewer. Interestingly, we found seven unique leporine amino acids, and the change of unique leporine amino acids with those conserved among other species, including S175N, Q221K, Q221R, A226Y, A230G, and A230S, was predicted to reduce hydrogen bonds in leporine PrP.</p>