AUTHOR=Rank Kaitlyn , Lynch Alex M. , Ruterbories Laura K. , Li Ronald H. L. , Ueda Yu 

TITLE=Evaluation of thrombin generation in dogs administered clopidogrel

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1194242

DOI=10.3389/fvets.2023.1194242

ISSN=2297-1769

ABSTRACT=<sec><title>Introduction</title><p>The antiplatelet effect of clopidogrel can vary between patients. A modified thromboelastography (TEG) protocol (TEG-Platelet Mapping assay<sup>®</sup> [TEG-PM]) can be used for clopidogrel monitoring but is not widely available. Thrombin generation (TG) assays could offer a novel alternative. The main objective of this pilot study was to assess TG assay variables (lag time, peak, endogenous thrombin potential [ETP]) in dogs before and after 7 days of clopidogrel administration and compare with TEG-PM variables (maximum amplitude [MA]-ADP and percentage (%) inhibition).</p></sec><sec><title>Methods</title><p>Six healthy mix-breed dogs were enrolled in this pilot study. Blood samples for platelet count, TG assays, and TEG-PM were obtained at two time points, corresponding to baseline, and after 7 days of clopidogrel administration (mean 2.3 +/− 0.3 mg/kg PO q24 hours). Data were then compared with a Student’s <italic>t</italic>-test.</p></sec><sec><title>Results</title><p>There was no significant change in TG assay variables performed on platelet poor plasma after 7 days of clopidogrel administration: lag time (Day 1: 1.8 +/− 0.2 min, Day 7: 1.8 +/− 0.2 min, <italic>p</italic> = 0.42); peak (Day 1: 76 +/− 7 nM, Day 7: 72 +/− 10 nM, <italic>p</italic> = 0.49); and ETP (Day 1: 399 +/− 27 nM*min, Day 7: 392 +/− 32 nM*min; <italic>p</italic> = 0.49). There were significant changes in TEG MA-ADP (Day 1: 19 +/− 8 mm, Day 7: 9 +/− 6 mm, <italic>p</italic> = 0.04) and % inhibition (Day 1: 58 +/− 27, Day 7: 99 +/− 0.3, <italic>p</italic> = 0.02).</p></sec><sec><title>Discussion</title><p>Clopidogrel administration did not lead to changes in TG assay variables performed on platelet poor plasma samples, despite concomitant changes in TEG-PM variables consistent with platelet inhibition. Based on this pilot study, thrombin generation performed on platelet poor plasma may not be a useful antiplatelet monitoring tool in dogs.</p></sec>