AUTHOR=Mao Yanni , Wang Yuxia , Luo Xiaofeng , Chen Xiaohui , Wang Guiqin 

TITLE=Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1184989

DOI=10.3389/fvets.2023.1184989

ISSN=2297-1769

ABSTRACT=<sec><title>Introduction</title><p>A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work.</p></sec><sec><title>Methods</title><p>This study focused on the changes in biofilm activity and related metabolic pathways of <italic>S. aureus</italic> treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS).</p></sec><sec><title>Results</title><p>The findings demonstrated that the LAB-CFS treatment considerably slowed <italic>Staphylococcus aureus</italic> (<italic>S. aureus</italic>) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the <italic>S. aureus</italic> biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of <italic>S. aureus</italic> biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways.</p></sec><sec><title>Discussion</title><p>These findings show that LAB-CFS has a strong potential to combat <italic>S. aureus</italic> infections.</p></sec>