AUTHOR=Barber Renee M. , Platt Simon R. , De Risio Luisa , Barber Jamie , Robinson Kelsey R. 

TITLE=Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1169617

DOI=10.3389/fvets.2023.1169617

ISSN=2297-1769

ABSTRACT=<sec><title>Introduction</title><p>Neuroinflammation that occurs in the brain after stroke has been shown to be important to disease pathogenesis and outcomes. The aim of this study was to evaluate a large number of pro- and anti-inflammatory cytokines in dogs with clinically-confirmed, naturally occurring stroke.</p></sec><sec><title>Materials and methods</title><p>Fifteen dogs with a clinical diagnosis of ischemic stroke and ten healthy control dogs were included in the study. A multiplex immunoassay was utilized to evaluate cerebrospinal fluid for GM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, CXCL1, MCP-1, and TNF-α.</p></sec><sec><title>Results</title><p>Mean concentrations of CXCL1 (stroke-436 pg/ml, control-267 pg/ml, <italic>p</italic> = 0.01) and MCP-1 (stroke-196 pg/ml, control-66 pg/ml, <italic>p</italic> ≤ 0.0001) were significantly elevated in dogs with stroke when compared with control dogs. Location and type of infarct, duration of clinical signs, and use of anti-inflammatory medications were not associated with differences in cytokine concentration.</p></sec><sec><title>Discussion</title><p>CXCL1 and MCP-1 may play a role in naturally occurring canine stroke and represent targets for future research.</p></sec>