AUTHOR=Keve Gergő , Csörgő Tibor , Benke Anikó , Huber Attila , Mórocz Attila , Németh Ákos , Kalocsa Béla , Tamás Enikő Anna , Gyurácz József , Kiss Orsolya , Kováts Dávid , Sándor Attila D. , Karcza Zsolt , Hornok Sándor 

TITLE=Ornithological and molecular evidence of a reproducing Hyalomma rufipes population under continental climate in Europe

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1147186

DOI=10.3389/fvets.2023.1147186

ISSN=2297-1769

ABSTRACT=<p>Reports on adult <italic>Hyalomma</italic> ticks in certain regions of the Carpathian Basin date back to the 19th century. These ticks were thought to emerge from nymphs dropping from birds, then molting to adults. Although the role of migratory birds in carrying ticks of this genus is known from all parts of Europe, in most countries no contemporaneous multiregional surveillance of bird-associated ticks was reported which could allow the recognition of hotspots in this context. From 38 passeriform bird species 956 ixodid ticks were collected at seven locations in Hungary. Tick species were identified as <italic>Ixodes ricinus</italic> (<italic>n</italic> = 598), <italic>Ixodes frontalis</italic> (<italic>n</italic> = 18), <italic>Ixodes lividus</italic> (<italic>n</italic> = 6), <italic>Haemaphysalis concinna</italic> (<italic>n</italic> = 321), and <italic>D. reticulatus</italic> (<italic>n</italic> = 1). All 12 <italic>Hyalomma</italic> sp. ticks (11 engorged nymphs and 1 unengorged larva) were identified as <italic>H. rufipes</italic> based on three mitochondrial markers. This species was only found in the Transdanubian region and along its southeastern border. The Common Blackbird and the European Robin were the two main hosts of <italic>I. ricinus</italic> and <italic>I. frontalis</italic>, whereas <italic>H. concinna</italic> was almost exclusively collected form long-distance migrants. The predominant hosts of <italic>H. rufipes</italic> were reed-associated bird species, the Sedge Warbler and the Bearded Reedling, both in their nesting period. This study provides ornithological explanation for the regional, century-long presence of adult <italic>Hyalomma</italic> ticks under continental climate in the Transdanubian Region of the Carpathian Basin. More importantly, the autochthonous occurrence of a <italic>H. rufipes</italic> population was revealed for the first time in Europe, based on the following observations: (1) the bird species infested with <italic>H. rufipes</italic> are not known to migrate during their nesting period; (2) one larva was not yet engorged; (3) the larva and the nymphs must have belonged to different local generations; and (4) all <italic>H. rufipes</italic> found in the relevant location were identical in their haplotypes based on three maternally inherited mitochondrial markers, probably reflecting founder effect. This study also demonstrated regional and temporal differences in tick species carried by birds.</p>