AUTHOR=Suárez-Vega Aroa , Frutos Pilar , Gutiérrez-Gil Beatriz , Esteban-Blanco Cristina , Toral Pablo G. , Arranz Juan-José , Hervás Gonzalo 

TITLE=Feed efficiency in dairy sheep: An insight from the milk transcriptome

JOURNAL=Frontiers in Veterinary Science

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1122953

DOI=10.3389/fvets.2023.1122953

ISSN=2297-1769

ABSTRACT=<sec><title>Introduction</title><p>As higher feed efficiency in dairy ruminants means a higher capability to transform feed nutrients into milk and milk components, differences in feed efficiency are expected to be partly linked to changes in the physiology of the mammary glands. Therefore, this study aimed to determine the biological functions and key regulatory genes associated with feed efficiency in dairy sheep using the milk somatic cell transcriptome.</p></sec><sec><title>Material and methods</title><p>RNA-Seq data from high (H-FE, <italic>n</italic> = 8) and low (L-FE, <italic>n</italic> = 8) feed efficiency ewes were compared through differential expression analysis (DEA) and sparse Partial Least Square-Discriminant analysis (sPLS-DA).</p></sec><sec><title>Results</title><p>In the DEA, 79 genes were identified as differentially expressed between both conditions, while the sPLS-DA identified 261 predictive genes [variable importance in projection (VIP) &gt; 2] that discriminated H-FE and L-FE sheep.</p></sec><sec><title>Discussion</title><p>The DEA between sheep with divergent feed efficiency allowed the identification of genes associated with the immune system and stress in L-FE animals. In addition, the sPLS-DA approach revealed the importance of genes involved in cell division (e.g., <italic>KIF4A</italic> and <italic>PRC1</italic>) and cellular lipid metabolic process (e.g., <italic>LPL, SCD, GPAM</italic>, and <italic>ACOX3</italic>) for the H-FE sheep in the lactating mammary gland transcriptome. A set of discriminant genes, commonly identified by the two statistical approaches, was also detected, including some involved in cell proliferation (e.g., <italic>SESN2, KIF20A</italic>, or <italic>TOP2A</italic>) or encoding heat-shock proteins (<italic>HSPB1</italic>). These results provide novel insights into the biological basis of feed efficiency in dairy sheep, highlighting the informative potential of the mammary gland transcriptome as a target tissue and revealing the usefulness of combining univariate and multivariate analysis approaches to elucidate the molecular mechanisms controlling complex traits.</p></sec>