AUTHOR=Karimi Poorya , Shafaghi-Sisi Soheila , Meamar Ahmad Reza , Nasiri Gelareh , Razmjou Elham 

TITLE=Prevalence and Molecular Characterization of Toxoplasma gondii and Toxocara cati Among Stray and Household Cats and Cat Owners in Tehran, Iran

JOURNAL=Frontiers in Veterinary Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.927185

DOI=10.3389/fvets.2022.927185

ISSN=2297-1769

ABSTRACT=<p><italic>Toxoplasma gondii</italic> and <italic>Toxocara</italic> spp. are the most critical parasites common between humans and cats. The close association of cats with humans in urban areas persuaded us to investigate the prevalence of these parasites in stray and household cats and their possible role in the owners' infection. Herein, 132 and 33 fecal samples of stray and household cats, respectively, and 33 blood samples of their owners were collected in Tehran, Iran. The prevalence of <italic>T. gondii</italic> was determined by targeting the <italic>B1</italic> gene in the feces of stray and household cats and the blood of cat owners. Furthermore, genotypes of <italic>T. gondii</italic> were identified based on the multilocus genotyping of BTUB, GRA6, SAG3, and APICO loci. <italic>Toxocara</italic> spp. were detected by targeting the second internal transcribed spacer (ITS-2) of the ribosomal DNA of these parasites in the cats' feces and the humans' blood. Also, <italic>Toxocara</italic> IgG was assessed in the human serum samples. The <italic>B1</italic> gene amplification showed that 15.2% of stray cats, 18.2% of household cats, and 51.5% of cat owners were infected with <italic>T. gondii</italic>. The multilocus sequence analysis revealed the predominance of genotype I of <italic>T. gondii</italic> in stray cats and genotype II of <italic>T. gondii</italic> in household cats and cat owners. The amplifying of ITS-2 revealed a high prevalence of <italic>T. cati</italic> infection (47.0%) in stray cats, whereas no infection was found in the feces of household cats or the serum of cat owners. Likewise, <italic>Toxocara</italic> IgG was not detected in the serum of humans. The lower prevalence of <italic>T. gondii</italic> in stray/household cats than in the cat owners indicates the limited impact of close contact with infected cats in human toxoplasmosis. However, the high prevalence of <italic>T. cati</italic> infection in stray cats can cause contamination of the environment by excreting eggs that may lead to infecting humans through soil or water. Therefore, public health education in urban management planning is necessary for routine urban cat deworming programs and for training the healthcare workers to prevent, control, and treat these infections.</p>