AUTHOR=Belij-Rammerstorfer Sandra , Limon Georgina , Maze Emmanuel A. , Hannant Kayleigh , Hughes Ellen , Tchakarova Simona R. , Alexandrov Tsviatko , Mmbaga Blandina T. , Willett Brian , Booth George , Lyons Nicholas A. , Baker Natalie , Thomas Kelly M. , Wright Daniel , Saunders Jack , Browning Clare , Wilsden Ginette , Carroll Miles , Hewson Roger , Charleston Bryan , Lambe Teresa , Ludi Anna B. TITLE=Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera JOURNAL=Frontiers in Veterinary Science VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.913046 DOI=10.3389/fvets.2022.913046 ISSN=2297-1769 ABSTRACT=

Crimean-Congo hemorrhagic fever (CCHF) is a priority emerging disease. CCHF, caused by the CCHF virus (CCHFV), can lead to hemorrhagic fever in humans with severe cases often having fatal outcomes. CCHFV is maintained within a tick-vertebrate-tick cycle, which includes domestic animals. Domestic animals infected with CCHFV do not show clinical signs of the disease and the presence of antibodies in the serum can provide evidence of their exposure to the virus. Current serological tests are specific to either one CCHFV antigen or the whole virus antigen. Here, we present the development of two in-house ELISAs for the detection of serum IgG that is specific for two different CCHFV antigens: glycoprotein Gc (CCHFV Gc) and nucleoprotein (CCHFV NP). We demonstrate that these two assays were able to detect anti-CCHFV Gc-specific and anti-CCHFV NP-specific IgG in sheep from endemic CCHFV areas with high specificity, providing new insight into the heterogeneity of the immune response induced by natural infection with CCHFV in domestic animals.