AUTHOR=Wei Dawei , Raza Sayed Haidar Abbas , Wang Xingping , Khan Rajwali , Lei Zhaoxiong , Zhang Guijie , Zhang Jiupan , Luoreng Zhuoma , Ma Yun , Alamoudi Muna O. , Aloufi Bandar Hamad , Alshammari Ahmed Mohajja , Abd El-Aziz Ayman Hassan , Alhomrani Majid , Alamri Abdulhakeem S. 

TITLE=Tissue Expression Analysis, Cloning, and Characterization of the 5′-Regulatory Region of the Bovine LATS1 Gene

JOURNAL=Frontiers in Veterinary Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.853819

DOI=10.3389/fvets.2022.853819

ISSN=2297-1769

ABSTRACT=<p>As a member of the large tumor suppressor (<italic>LATS</italic>) gene family, <italic>LATS</italic>1 plays an important role in regulating muscle growth and development. In this study, we determined the distinct exhibit patterns of tissue expression of bovine <italic>LATS</italic>1. Further, we determined the functional proximal minimal promoter of bovine <italic>LATS</italic>1 and identified the key transcription factors in the core promoter region to elucidate its molecular regulation mechanism. The results showed that bovine <italic>LATS</italic>1 was highly expressed in the <italic>longissimus thoracis</italic> and upregulation in infancy muscle. An electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay in combination with site-directed mutation and small interfering RNA (siRNA) interference demonstrated that myogenic differentiation 1 (Myod1) and myocyte enhancer factor 2A (MEF2A) binding in the core promoter region (−298/−123 bp) play important roles in the transcriptional regulation of the bovine <italic>LATS</italic>1 promoter. Taken together, these interactions provide insight into the regulatory mechanisms of <italic>LATS</italic>1 transcription in mediating skeletal muscle growth in cattle.</p>