AUTHOR=Chen Xiabing , Shao Zhiyong , Wu Lijun , He Bin , Yang Wenhai , Chen Jie , Jin Erguang , Huang Qi , Lei Liancheng , Xu Jiajia , Li Haotian , Zhang Hui , Wan Yun , Liu Wu , Zhou Rui 

TITLE=Involvement of the Actinobacillus pleuropneumoniae ompW Gene in Confrontation of Environmental Pressure

JOURNAL=Frontiers in Veterinary Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.846322

DOI=10.3389/fvets.2022.846322

ISSN=2297-1769

ABSTRACT=<p>Actinobacillus pleuropneumoniae causes porcine pleuropneumonia. The function of the outer membrane protein W gene (<italic>ompW</italic>) of <italic>A. pleuropneumoniae</italic> has not been evaluated. Thus a deletion mutant of <italic>ompW</italic>, Δ<italic>ompW</italic>, was constructed to explore the effect of <italic>ompW</italic> gene deletion on bacterial growth, biofilm formation, bacterial morphology, oxidative tolerance, susceptibility to antibiotics, and the expression of ribosome synthesis and ABC transporter related genes. Results showed that the <italic>ompW</italic> gene deletion did not affect biofilm formation and the growth of <italic>A. pleuropneumoniae</italic> but did affect bacterial morphology during steady growth, oxidative tolerance, and bacterial susceptibility to polymyxin B, kanamycin, and penicillin. The <italic>ompW</italic> gene deletion also affected the expression of ribosome synthesis and ABC transporter related genes. These results suggested that <italic>ompW</italic> may regulate the biological phenotype of <italic>A. pleuropneumoniae</italic>.</p>