AUTHOR=Zhao Mengmeng , Li Huawei , Zhang Hang , Sha Huiyang , Huang Liangzong , Wang Ruining 

TITLE=Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway

JOURNAL=Frontiers in Veterinary Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.805301

DOI=10.3389/fvets.2022.805301

ISSN=2297-1769

ABSTRACT=<p>Monkey diseases are becoming increasingly severe, and some may be transmitted to humans through direct and indirect contact. Innate immunity is the first line of defense against foreign microorganisms. It is of great significance to explore the immune characteristics of monkey and human diseases. TRIM4, an important immune molecule in <italic>Macaca mulatta</italic>, was cloned and its immunological characteristics were preliminarily explored. The results showed that <italic>Macaca mulatta TRIM4</italic> was in the same branch as human <italic>TRIM4</italic>. Overexpression of TRIM4 increased the mRNA levels of interferon (IFN)-<italic>alpha, IFN-beta, RIG-I, MAVS, IRF3, IRF7, OAS1, IFIT3, and CCL5</italic>, TRIM4 up-regulated the activities of IFN-beta, NF-κB, and ISRE reporter. In contrast, inhibiting <italic>TRIM4</italic> expression by small interfering RNA (siRNA) down-regulated the IFN pathway. In summary, <italic>Macaca mulatta TRIM4</italic> plays an essential role in the IFN pathway.</p>