AUTHOR=Zhou Yonghui , Yu Fei , Chen Mo , Zhang Yuefeng , Qu Qianwei , Wei Yanru , Xie Chunmei , Wu Tong , Liu Yanyan , Zhang Zhiyun , Chen Xueying , Dong Chunliu , Che Ruixiang , Li Yanhua 

TITLE=Tylosin Inhibits Streptococcus suis Biofilm Formation by Interacting With the O-acetylserine (thiol)-lyase B CysM

JOURNAL=Frontiers in Veterinary Science

VOLUME=8

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2021.829899

DOI=10.3389/fvets.2021.829899

ISSN=2297-1769

ABSTRACT=<p><italic>Streptococcus suis</italic> (<italic>S. suis</italic>) can decrease its virulence or modify local conditions through biofilm formation, which promotes infection persistence <italic>in vivo</italic>. Biofilm formation is an important cause of chronic drug-resistant <italic>S. suis</italic> infection. The aim of this study was to evaluate whether tylosin effectively inhibits <italic>S. suis</italic> biofilm formation by interacting with O-acetylserine (thiol)-lyase B (CysM), a key enzymatic regulator of cysteine synthesis. Biofilm formation of the mutant (ΔcysM) strain was significantly lower compared to the wild-type ATCC 700794 strain. Tylosin inhibited <italic>cysM</italic> gene expression, decreased extracellular matrix contents, and reduced cysteine, homocysteine, and S-adenosylmethionine levels, indicating its potential value as an effective inhibitor of <italic>S. suis</italic> biofilm formation. Furthermore, using biolayer interferometry technology and fourier-transform infrared spectroscopy, we found that tylosin and CysM could be combined directly. Overall, these results provide evidence that tylosin inhibits <italic>S. suis</italic> biofilm formation by interacting with CysM.</p>