AUTHOR=Bai Yanbin , Li Xupeng , Chen Zongchang , Li Jingsheng , Tian Hongshan , Ma Yong , Raza Sayed Haidar Abbas , Shi Bingang , Han Xiangmin , Luo Yuzhu , Hu Jiang , Wang Jiqing , Liu Xiu , Li Shaobin , Zhao Zhidong 

TITLE=Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

JOURNAL=Frontiers in Veterinary Science

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2021.788316

DOI=10.3389/fvets.2021.788316

ISSN=2297-1769

ABSTRACT=<p>The enzyme long-chain acyl-CoA synthetase 1 (ACSL1) is essential for lipid metabolism. The <italic>ACSL1</italic> gene controls unsaturated fatty acid (UFA) synthesis as well as the formation of lipid droplets in bovine adipocytes. Here, we used RNA-Seq to determine lncRNA and mRNA that regulate UFA synthesis in bovine adipocytes using RNA interference and non-interference with <italic>ACSL1</italic>. The corresponding target genes of differentially expressed (DE) lncRNAs and the DE mRNAs were found to be enriched in lipid and FA metabolism-related pathways, according to GO and KEGG analyses. The differentially expressed lncRNA- differentially expressed mRNA (DEL-DEM) interaction network indicated that some DELs, such as TCONS_00069661, TCONS_00040771, TCONS_ 00035606, TCONS_00048301, TCONS_001309018, and TCONS_00122946, were critical for UFA synthesis. These findings assist our understanding of the regulation of UFA synthesis by lncRNAs and mRNAs in bovine adipocytes.</p>