AUTHOR=Peng Zhong , Liu Junyang , Liang Wan , Wang Fei , Wang Li , Wang Xueying , Hua Lin , Chen Huanchun , Wilson Brenda A. , Wang Jia , Wu Bin 

TITLE=Development of an Online Tool for Pasteurella multocida Genotyping and Genotypes of Pasteurella multocida From Different Hosts

JOURNAL=Frontiers in Veterinary Science

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2021.771157

DOI=10.3389/fvets.2021.771157

ISSN=2297-1769

ABSTRACT=<p><italic>Pasteurella multocida</italic> is a versatile zoonotic pathogen. Multiple systems have been applied to type <italic>P. multocida</italic> from different diseases in different hosts. Recently, we found that assigning <italic>P. multocida</italic> strains by combining their capsular, lipopolysaccharide, and MLST genotypes (marked as capsular: lipopolysaccharide: MLST genotype) could help address the biological characteristics of <italic>P. multocida</italic> circulation in different hosts. However, there is still lack of a rapid and efficient tool to diagnose <italic>P. multocida</italic> according to this system. Here, we developed an intelligent genotyping platform PmGT for <italic>P. multocida</italic> strains according to their whole genome sequences using the web 2.0 technologies. By using PmGT, we determined capsular genotypes, LPS genotypes, and MLST genotypes as well as the main virulence factor genes (VFGs) of <italic>P. multocida</italic> isolates from different host species based on their whole genome sequences published on NCBI. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. With the advent of high-quality, inexpensive DNA sequencing, PmGT represents a more efficient tool for <italic>P. multocida</italic> diagnosis in both epidemiological studies and clinical settings.</p>